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Literature summary for 1.14.16.1 extracted from

  • Miranda, F.F.; Kolberg, M.; Andersson, K.K.; Geraldes, C.F.; Martinez, A.
    The active site residue tyrosine 325 influences iron binding and coupling efficiency in human phenylalanine hydroxylase (2005), J. Inorg. Biochem., 99, 1320-1328.
    View publication on PubMed
Search for more literature for 1.14.16.1

Protein Variants

Protein Variants Comment Organism
Y325A high degree of aggregation, but suffivcient tetrameric form for characterization Homo sapiens
Y325F stable, similar yields and oligomeric distribution as wild-type, posttranslational hydroxylation Homo sapiens
Y325L stable, similar yields and oligomeric distribution as wild-type, reduced specific activity, decreased coupling efficiency and decreased iron content, no positive cooperativity for L-phenylalanine Homo sapiens
Y325S aggregation after purification, not suitable for characterization Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 Abz-VAA mutant Y325L, 25°C, pH 7.0 Homo sapiens
0.008
-
 Abz-VAA mutant enzyme Y325L, 25°C, pH 7.0 Homo sapiens
0.027
-
 Abz-VAA mutant Y325F, 25°C, pH 7.0 Homo sapiens
0.027
-
 Abz-VAA mutant enzyme Y325F, 25°C, pH 7.0 Homo sapiens
0.028
-
 Abz-VAA wild-type, 25°C, pH 7.0 Homo sapiens
0.028
-
 Abz-VAA wild type enzyme, 25°C, pH 7.0 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
290
-
 mutant Y325A, 25°C, pH 7.0 Homo sapiens
300
-
 mutant Y325A, preincubation with L-phenylalanine, 25°C, pH 7.0 Homo sapiens
1150
-
 mutant Y325F, 25°C, pH 7.0 Homo sapiens
1230
-
 wild-type, 25°C, pH 7.0 Homo sapiens
1310
-
 mutant Y325L, 25°C, pH 7.0 Homo sapiens
1500
-
 mutant Y325L, preincubation with L-phenylalanine, 25°C, pH 7.0 Homo sapiens
3630
-
 mutant Y325F, preincubation with L-phenylalanine, 25°C, pH 7.0 Homo sapiens
3640
-
 wild-type, preincubation with L-phenylalanine, 25°C, pH 7.0 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-VAA + tetrahydrobiopterin + O2
-
 Homo sapiens ?
-
 r
L-phenylalanine + tetrahydrobiopterin + O2
-
 Homo sapiens L-tyrosine + dihydrobiopterin + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin
-
 Homo sapiens