Literature summary for 1.14.16.1 extracted from

Gamez, A.; Perez, B.; Ugarte, M.; Desviat, L.R.
Expression analysis of phenylketonuria mutations: effect on folding and stability of the phenylalanine hydroxylase protein (2000), J. Biol. Chem., 275, 29737-29742.

Search for more literature for 1.14.16.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type, L348V, L349L and V388M mutant enzyme maltose-binding-protein fusions in Escherichia coli and COS cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
L348V	25% activity after expression in Escherichia coli in the absence of GroESL, 55% in the presence of GroESL, 77% activity after expression in COS cells at 27°C	Homo sapiens
S348L	instable enzyme forming aggregates after expression in Escherichia coli in the presence of GroESL	Homo sapiens
V388M	40% activity after expression in Escherichia coli in the absence of GroESL, 82% in the presence of GroESL, 78% activity after expression in COS cells at 27°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P00439	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type, L348V and V388M mutant enzyme, affinity chromatography	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	V388M mutant enzyme, 50% activity after 10 min	Homo sapiens
51	-	L348V mutant enzyme, 50% activity after 10 min	Homo sapiens
59	-	recombinant wild-type enzyme, 50% activity after 10 min	Homo sapiens

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Release 2023.1 (January 2023)