Literature summary for 1.14.16.1 extracted from

Knappskog, P.M.; Flatmark, T.; Aarden, J.M.; Haavik, J.; Martinez, A.
Structure/function relationships in human phenylalanine hydroxylase. Effect of terminal deletions on the oligomerization, activation and cooperativity of substrate binding to the enzyme (1996), Eur. J. Biochem., 242, 813-821.

Search for more literature for 1.14.16.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type enzyme and Asp112-Lys452, Ser2-Gln428 and Gly103-Gln428 deletion mutants in Escherichia coli	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.021	-	tetrahydrobiopterin	recombinant wild-type enzyme, dimeric form	Homo sapiens
0.025	-	tetrahydrobiopterin	recombinant wild-type enzyme, tetrameric form	Homo sapiens
0.031	-	tetrahydrobiopterin	Gly103-Gln428 deletion mutant, dimeric form	Homo sapiens
0.032	-	tetrahydrobiopterin	Ser2-Gln428 deletion mutant, dimeric form	Homo sapiens
0.034	-	tetrahydrobiopterin	Asp112-Lys452 deletion mutant, tetrameric form	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type enzyme and deletion mutants	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0183	-	phenylalanine	Ser2-Gln428 deletion mutant, tetrameric form	Homo sapiens
0.135	-	phenylalanine	Ser2-Gln428 deletion mutant, L-phenylalanine activated, dimeric form	Homo sapiens
0.165	-	phenylalanine	Ser2-Gln428 deletion mutant, lysophosphatidylcholine activated, dimeric form	Homo sapiens
0.233	-	phenylalanine	Ser2-Gln428 deletion mutant, lysophosphatidylcholine and phenylalanine activated, dimeric form	Homo sapiens
0.363	-	phenylalanine	recombinant enzyme, tetrameric form	Homo sapiens
0.45	-	phenylalanine	Asp112-Lys452 deletion mutant, lysophosphatidylcholine activated, tetrameric form	Homo sapiens
0.5	-	phenylalanine	Asp112-Lys452 deletion mutant, tetrameric form	Homo sapiens
0.667	-	phenylalanine	Gly103-Gln428 deletion mutant, lysophosphatidylcholine activated, dimeric form	Homo sapiens
0.877	-	phenylalanine	recombinant enzyme, lysophosphatidylcholine activated, tetrameric form	Homo sapiens
0.952	-	phenylalanine	Gly103-Gln428 deletion mutant, tetrameric form	Homo sapiens
1.05	-	phenylalanine	Asp112-Lys452 deletion mutant, L-phenylalanine activated, tetrameric form	Homo sapiens
1.05	-	phenylalanine	Asp112-Lys452 deletion mutant, lysophosphatidylcholine and phenylalanine activated, tetrameric form	Homo sapiens
1.24	-	phenylalanine	Gly103-Gln428 deletion mutant, lysophosphatidylcholine and phenylalanine activated, dimeric form	Homo sapiens
1.3	-	phenylalanine	recombinant enzyme, L-phenylalanine activated, tetrameric form	Homo sapiens
1.33	-	phenylalanine	Gly103-Gln428 deletion mutant, L-phenylalanine activated, dimeric form	Homo sapiens
1.59	-	phenylalanine	recombinant enzyme, lysophosphatidylcholine and phenylalanine activated, tetrameric form	Homo sapiens

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Release 2023.1 (January 2023)