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Literature summary for 1.14.16.1 extracted from

  • Letendre, C.H.; Dickens, G.; Guroff, G.
    Phenylalanine hydroxylase from Pseudomonas sp. (ATCC 11299a). Purification, molecular weight, and influence of tyrosine metabolites on activation and hydroxylation (1975), J. Biol. Chem., 250, 6672-6678.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
4-hydroxyphenylacetic acid 0.4 mM, approx. 30% activation Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxyphenylpyruvic acid above 0.4 mM iron, activation below Pseudomonas sp.
D,L-DOPA 0.1 mM, approx. 60% inhibition Pseudomonas sp.
D,L-m-tyrosine 0.4 mM, approx. 80% inhibition Pseudomonas sp.
dopamine 0.5 mM, approx. 40% inhibition Pseudomonas sp.
L-3,4-dihydroxyphenylalanine
-
 Pseudomonas sp.
L-Dopa 0.3 mM, approx. 40% inhibition Pseudomonas sp.
L-tryptophan
-
 Pseudomonas sp.
phenylalanine
-
 Pseudomonas sp.
tyrosine
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000 27000 sucrose density gradient centrifugation, gel filtration Pseudomonas sp.
28000
-
 1 * 28000, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.9
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2
-
 Pseudomonas sp. L-tyrosine + dihydrobiopterin + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
tetrahydrobiopterin
-
 Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.11
-
 phenylalanine competitive vs. iron Pseudomonas sp.
0.3
-
 L-tryptophan competitive vs. iron Pseudomonas sp.
0.3
-
 tyrosine competitive vs. iron Pseudomonas sp.