Crystallization (Comment) | Organism |
---|---|
vapour diffusion, 1 mg enzyme dissolved in 0.1 ml 35% ammonium sulfate, 50 mM acetate, pH 6.0, 1 mM dithiothreitol, reservoir contains 60% ammonium sulfate, crystals appear after 3-4 d at 4°C | Chromobacterium violaceum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine | - |
Chromobacterium violaceum | |
0.14 | - |
L-phenylalanine | - |
Chromobacterium violaceum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | 32400 | gel filtration, SDS-PAGE, sedimentation studies | Chromobacterium violaceum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Chromobacterium violaceum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.2 | - |
- |
Chromobacterium violaceum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine | - |
Chromobacterium violaceum | ? | - |
r | |
L-phenylalanine + tetrahydrobiopterin + O2 | additional electron donors: 6-methylpterin, 6,7-dimethyltetrahydropterin | Chromobacterium violaceum | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | additional electron donors: 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, 2-amino-4-hydroxy-6-methyltetrahydropteridine, tetrahydrofolate, and 6-methyl-5-deazatetrahydropterin | Chromobacterium violaceum | L-tyrosine + dihydrobiopterin + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | artificial cofactors: 6-methyltetrahydropterin, 6,7-dimethyltetrahydropterin | Chromobacterium violaceum | |
tetrahydrobiopterin | - |
Chromobacterium violaceum | |
tetrahydrofolate | 10% of activity with 6,7-dimethyltetrahydropterin | Chromobacterium violaceum |