Activating Compound | Comment | Organism | Structure |
---|---|---|---|
lysolecithin | - |
Rattus norvegicus | |
additional information | - |
Rattus norvegicus | |
phenylalanine | major regulator of liver enzyme, binds at an effector site converting the inactive to an active enzyme | Rattus norvegicus | |
Phospholipids | activate | Rattus norvegicus |
General Stability | Organism |
---|---|
frozen and thawed enzyme is 10-20% less active than before freezing | Rattus norvegicus |
glycerol + EDTA stabilize | Rattus norvegicus |
NaCl or KCl above 200 mM stabilize | Rattus norvegicus |
nonionic detergents e.g. Triton X-100 and Tween 80, 0.03-0.1%, stabilize | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(6R)-L-erythro-tetrahydrobiopterin | inhibits phenylalanine and lysolecithin activation | Rattus norvegicus | |
catechol | - |
Rattus norvegicus | |
glycerol | - |
Rattus norvegicus | |
H2O2 | inactivates the reduced form of the enzyme | Rattus norvegicus | |
Tris | - |
Rattus norvegicus | |
Tween 80 | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | 0.015 | tetrahydrobiopterin | substrate phenylalanine | Rattus norvegicus | |
0.045 | - |
6-methyltetrahydropterin | substrate phenylalanine | Rattus norvegicus | |
0.18 | - |
phenylalanine | cofactor 6-methyltetrahydropterin | Rattus norvegicus | |
0.2 | - |
phenylalanine | cofactor tetrahydrobiopterin | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.5 | 13 | - |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, 2 years, no loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | additional electron donors: 6-methylpterin, 6,7-dimethyltetrahydropterin | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | additional electron donors: 6-methyltetrahydropterin, 7-methylpterin, and 2,4,5-triamino-6-hydroxypyrimidine | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | artificial cofactors: 6-methyltetrahydropterin, 6,7-dimethyltetrahydropterin | Rattus norvegicus | |
additional information | artificial cofactors: 6-methyltetrahydropterin, 7-methylpterin | Rattus norvegicus | |
tetrahydrobiopterin | - |
Rattus norvegicus |