Activating Compound | Comment | Organism | Structure |
---|---|---|---|
lysolecithin | - |
Rattus norvegicus | |
additional information | - |
Rattus norvegicus | |
Phospholipids | activate | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridine | - |
Rattus norvegicus | |
8-hydroxyquinoline | - |
Rattus norvegicus | |
catechol | - |
Rattus norvegicus | |
diethyldithiocarbamate | - |
Rattus norvegicus | |
Dopa | - |
Rattus norvegicus | |
dopamine | - |
Rattus norvegicus | |
halogenated phenylalanine | moderate | Rattus norvegicus | |
additional information | not inhibited by EDTA | Rattus norvegicus | |
norepinephrine | - |
Rattus norvegicus | |
o-phenanthroline | - |
Rattus norvegicus | |
S-methyl-L-cysteine | 6 mM, 55% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | 0.004 | tetrahydrobiopterin | - |
Rattus norvegicus | |
0.05 | 0.06 | 6,7-dimethyltetrahydropterin | - |
Rattus norvegicus | |
0.2 | - |
phenylalanine | cofactor tetrahydrobiopterin | Rattus norvegicus | |
0.47 | - |
thienylalanine | native enzyme | Rattus norvegicus | |
0.47 | - |
thienylalanine | native enzyme, cofactor tetrahydrobiopterin | Rattus norvegicus | |
1.3 | - |
phenylalanine | cofactor 6,7-dimethyltetrahydrobiopterin | Rattus norvegicus | |
1.7 | - |
thienylalanine | cofactor tetrahydrobiopterin, lysolecithin activated enzyme | Rattus norvegicus | |
1.7 | - |
thienylalanine | lysolecithin-activated enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-protein | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | 55000 | monomeric form, enzyme exists as monomer, dimer and tetramer | Rattus norvegicus |
100000 | 110000 | dimeric form, enzyme exists as monomer, dimer and tetramer | Rattus norvegicus |
200000 | 210000 | tetrameric species accounts for 78% of the total enzyme, enzyme exists as monomer, dimer and tetramer | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + (6R)-tetrahydrobiopterin + O2 | Rattus norvegicus | in mammals rate-limiting step in complete catabolism of phenylalanine to CO2 and water | L-tyrosine + (6R)-dihydrobiopterin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.59 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, 1 year, 30% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-fluorophenylalanine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
3-fluorophenylalanine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
3-phenylserine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
4-chlorophenylalanine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
4-fluorophenylalanine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
beta-2-thienylalanine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
L-methionine + tetrahydrobiopterin + O2 | lysolecithin activated enzyme | Rattus norvegicus | ? | - |
? | |
L-norleucine + tetrahydrobiopterin + O2 | lysolecithin activated enzyme | Rattus norvegicus | ? | - |
? | |
L-phenylalanine + (6R)-tetrahydrobiopterin + O2 | in mammals rate-limiting step in complete catabolism of phenylalanine to CO2 and water | Rattus norvegicus | L-tyrosine + (6R)-dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | additional electron donors: 6-methylpterin, 6,7-dimethyltetrahydropterin | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | 4-alpha-carbinolamine is the first free pterin product formed | ? | |
L-phenylalanine + tetrahydrobiopterin + O2 | specificity is quantitatively altered when the enzyme is activated by lysolecithin | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | 4-alpha-carbinolamine is the first free pterin product formed | ? | |
L-phenylalanine + tetrahydrobiopterin + O2 | additional electron donors: 6-methyltetrahydropterin, 7-methylpterin, and 2,4,5-triamino-6-hydroxypyrimidine | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | 4-alpha-carbinolamine is the first free pterin product formed | ? | |
L-phenylalanine + tetrahydrobiopterin + O2 | low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | 4-alpha-carbinolamine is the first free pterin product formed | ? | |
L-tryptophan + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
m-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
p-methylphenylalanine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
S-methyl-L-cysteine + tetrahydrobiopterin + O2 | lysolecithin activated enzyme | Rattus norvegicus | ? | - |
? | |
thienylalanine + tetrahydrobiopterin | - |
Rattus norvegicus | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | enzyme exists as monomer, dimer and tetramer | Rattus norvegicus |
monomer | enzyme exists as monomer, dimer and tetramer | Rattus norvegicus |
tetramer | enzyme exists as monomer, dimer and tetramer | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | artificial cofactors: 6-methyltetrahydropterin, 6,7-dimethyltetrahydropterin | Rattus norvegicus | |
additional information | artificial cofactor: 2,4,5-triamino-6-hydroxypyrimidine | Rattus norvegicus | |
additional information | artificial cofactors: 6-methyltetrahydropterin, 7-methylpterin | Rattus norvegicus | |
tetrahydrobiopterin | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
catechol | - |
Rattus norvegicus |