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Literature summary for 1.14.15.7 extracted from
- Isolation and characterization of a novel peroxisomal choline monooxygenase in barley (2011), Planta, 234, 1215-1226.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene HvCMO, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR expression analyis, recombinant expression in Saccharomyces cerevisiae strain BJ5458 | Hordeum vulgare |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | no activity in mitochondria and the endoplasmic reticulum-containing microsome fraction, as well as chloroplasts | Hordeum vulgare | - |
- |
| peroxisome | - |
Hordeum vulgare | 5777 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | in ferredoxin cofactor and a Rieske-type [2Fe-2S] cluster | Hordeum vulgare |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44800 | - |
x * 44800, about, sequence calculation | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + O2 + 2 reduced ferredoxin + 2 H+ | Hordeum vulgare | - |
betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | B5UAE1 | cv. Haruna-nijyo | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| awn | - |
Hordeum vulgare | - |
| floret | - |
Hordeum vulgare | - |
| internode | - |
Hordeum vulgare | - |
| leaf | high content of glycine betaine, more in the older leaf blades, but little in the florets and internodes | Hordeum vulgare | - |
| seedling | - |
Hordeum vulgare | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + O2 + 2 reduced ferredoxin + 2 H+ | - |
Hordeum vulgare | betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44800, about, sequence calculation | Hordeum vulgare |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CMO | - |
Hordeum vulgare |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Hordeum vulgare |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Fe-S center | Rieske-type [2Fe-2S] cluster | Hordeum vulgare | |
| Ferredoxin | dependent on | Hordeum vulgare |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Hordeum vulgare | sequence calculation | - |
6.2 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Hordeum vulgare | the expression of HvCMO protein is increased by the presence of high NaCl, accumulation of HvCMO mRNA is increased by high osmotic and low-temperature environments | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme catalyzes the first stepin biosynthesis of glycine betaine. The second step is catalyzed by betaine aldehyde dehydrogenase | Hordeum vulgare |
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