Literature summary for 1.14.15.6 extracted from

Lepesheva, G.I.; Azeva, T.N.; Strushkevich, N.V.; Gilep, A.A.; Usanov, S.A.
Site-directed mutagenesis of cytochrome P450scc (CYP11A1). Effect of lysine residue substitution on its structural and functional properties (2000), Biochemistry (Moscow), 65, 1409-1418.

Search for more literature for 1.14.15.6

Cloned(Commentary)

Cloned (Comment)	Organism
mutants and wild-type expressed in Escherichia coli	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
K103Q	decrease of stability	Bos taurus
K104Q	dramatic decrease in expression level	Bos taurus
K109Q	dramatic changes in folding and heme insertion	Bos taurus
K110Q	folding and heme insertion not affected	Bos taurus
K145Q	-	Bos taurus
K148Q	expression level is not affected, extreme instable and rapid denaturation	Bos taurus
K394Q	functional parameters decreased because substitution is close to 405 position, but to much lower extend	Bos taurus
K403Q	functional parameters decreased because substitution is close to 405 position, but to much lower extend, shows importance of this residue for electrostatic interaction with negatively charged residues of ferredoxin	Bos taurus
K405Q	dramatic loss of activity, shows importance of this residue for electrostatic interaction with negatively charged residues of ferredoxin	Bos taurus
additional information	site-directed mutagenesis, no evident changes in functional properties for KQ mutants, 103, 110, 145, 394 and 403 with activities between 69 and 86% of wild-type	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrial inner membrane	inner membrane	Bos taurus	5743	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cholesterol + 6 reduced adrenodoxin + 3 O2	Bos taurus	-	pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O	precursor of all steroid hormones	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
precipitation with polyethylene glycol, affinity chromatography	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
adrenal cortex	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cholesterol + 6 reduced adrenodoxin + 3 O2	-	Bos taurus	pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O	precursor of all steroid hormones	?
cholesterol + reduced adrenodoxin + O2	-	Bos taurus	pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O	-	?

Cofactor

Cofactor	Comment	Organism	Structure
adrenodoxin	-	Bos taurus
heme	-	Bos taurus
NADPH	NADPH-linked adrenodoxin reductase	Bos taurus

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Release 2023.1 (January 2023)