Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.15.6 extracted from

  • Takemori, S.; Sukara, K.; Hashimoto, K.; Hashimoto, M.; Sato, H.; Gomi, T.; Katagiri, M.
    Purification of cytochrome P-450 from bovine adrenocortical mitochondria by an aniline-Sepharose and the properties (1975), Biochem. Biophys. Res. Commun., 63, 588-593.
    View publication on PubMed
Search for more literature for 1.14.15.6

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane
-
 Bos taurus 5743
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 4 * 46000, sedimentation equilibrium analysis after guanidine treatment and SDS-PAGE Bos taurus
200000
-
 sedimentation equilibrium analysis Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
-80°C, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.01% cholate, a few days without substantial loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
additional information
-
 Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 46000, sedimentation equilibrium analysis after guanidine treatment and SDS-PAGE Bos taurus

Cofactor

Cofactor Comment Organism Structure
adrenodoxin
-
 Bos taurus
NADPH NADPH-linked adrenodoxin reductase Bos taurus