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Literature summary for 1.14.15.6 extracted from

  • Hanukoglu, I.; Spitsberg, V.; Bumpus, J.A.; Dus, K.M.; Jefcoate, C.R.
    Adrenal mitochondrial cytochrome P-450scc. Cholesterol and adrenodoxin interactions at equilibrium and during turnover (1981), J. Biol. Chem., 256, 4321-4328.
    View publication on PubMed
Search for more literature for 1.14.15.6

Activating Compound

Activating Compound Comment Organism Structure
Phospholipid cholesterol gains access to the cytochrome within micelles Bos taurus
Tween 20 small stabilizing and binding activating effect on ternary complex of adrenodoxin-cholesterol-P-450scc Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibits side chain cleavage activity optimally activated by 100 mM NaCl Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 reduced adrenodoxin
-
 Bos taurus
0.39
-
 cholesterol
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane adrenodoxin reductase and adrenodoxin are peripheral proteins on matrix side of inner membrane, P-450scc is integral membrane protein Bos taurus 5743
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ very little Bos taurus
Mg2+
-
 Bos taurus
additional information Kd for adrenodoxin increases with increase of ions from NaCl, binding of adrenodoxin to P-450scc is more sensitive to changes in metal ion concentration than cholesterol binding, high salt concentrations cause fall-off in activity Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractioning, aniline-Sepharose, adrenodoxin-Separose Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
-70°C, mitochondrial pellet, 30-40 mg/ml, 100 mM potassium phosphate, pH 7.3, 0.2 mM EDTA Bos taurus
4°C, purified, 50 mM potassium phosphate, pH 7.3, 0.1 mM dithiothreitol, 10% glycerol, stable a few weeks Bos taurus
purified, 25 mM potassium phosphate, pH 7.3, 0.05 mM EDTA, 0.05 mM dithiothreitol, 0.025 mM deoxycorticosterone, 0.25% Tween 20, 0.25% sodium cholate Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
cholesterol + reduced adrenodoxin + O2 + H+
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 r
additional information suggested shuttle mechanism in which adrenodoxin transfers electrons between adrenodoxin reductase and cytochrome P-450. No ternary adrenodoxin-adrenodoxin reductase-P-450 complex Bos taurus ?
-
 ?
additional information dissociation of oxidized adrenodoxin from P-450scc must be a potential rate-limiting factor Bos taurus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 increase of complex formation of adrenodoxin and cytochrome P-450scc in absence of cholesterol with decrease of temperature to 6°C, elevated temperature decreases the affinity of P-450scc for cholesterol Bos taurus

Cofactor

Cofactor Comment Organism Structure
adrenodoxin transports electrons from adrenodoxin reductase to cytochrome P-450 by shuttling Bos taurus
heme
-
 Bos taurus
NADPH NADPH-linked adrenodoxin reductase Bos taurus