Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.15.4 extracted from

  • Suzuki, K.; Sanga, K.i.; Chikaoka, Y.; Itagaki, E.
    Purification and properties of cytochrome P-450 (P-450lun) catalyzing steroid 11beta-hydroxylation in Curvularia lunata (1993), Biochim. Biophys. Acta, 1203, 215-223.
    View publication on PubMed
Search for more literature for 1.14.15.4

General Stability

General Stability Organism
11-deoxycortisol, partially stabilizes Curvularia lunata
glycerol, partially stabilizes Curvularia lunata
GSH, partially stabilizes Curvularia lunata
PMSF, partially stabilizes Curvularia lunata

Inhibitors

Inhibitors Comment Organism Structure
CO strongly Curvularia lunata
HgCl2 0.2 mM, 50% inhibition Curvularia lunata
ketoconazole
-
 Curvularia lunata
Metyrapone
-
 Curvularia lunata
PCMB 0.1 mM, 50% inhibition Curvularia lunata
sodium cholate 0.2% effect nearly 20% inhibition Curvularia lunata
Stilbestrol
-
 Curvularia lunata
sulfhydryl reagents strongly Curvularia lunata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information lipids such as DLPC required Curvularia lunata
0.077
-
 11-deoxycortisol 11beta-hydroxylation Curvularia lunata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Curvularia lunata

Organism

Organism UniProt Comment Textmining
Curvularia lunata
-
 Boedijn NRRL 2380
-

Purification (Commentary)

Purification (Comment) Organism
Triton X-100, sodium cholate, column chromatographies Curvularia lunata

Reaction

Reaction Comment Organism Reaction ID
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O one bifunctional cytochrome P-450, 11beta-/14alpha-hydroxylation Curvularia lunata
a

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Curvularia lunata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 dependent on DLPC concentration, with 0.05 mM maximum activity, 2fold increase with addition of 15% glycerol, 0.6 mM GSH, 0.01 mM FAD and 0.01 mM FMN Curvularia lunata

Storage Stability

Storage Stability Organism
-80°C, purified, 200 mM sodium phosphate buffer, pH 8.0, 0.2 mM 11-deoxycortisol, 20% glycerol, 0.1 mM GSH, 0.1% sodium cholate, several days without loss of activity Curvularia lunata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-deoxycortisol + reduced adrenal ferredoxin + O2 11beta-hydroxylase activity Curvularia lunata cortisol + oxidized adrenal ferredoxin + H2O
-
 ?
11-deoxycortisol + reduced adrenal ferredoxin + O2 14alpha-hydroxylase activity Curvularia lunata 14alpha-hydroxy-11-deoxycortisol + oxidized adrenal ferredoxin + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Curvularia lunata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 30 mM sodium phosphate buffer Curvularia lunata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 30 min, 30 mM sodium phosphate buffer, pH 8.0, decrease to 30%, improved to 45-70% by addition of 1 mM GSH, 0.2 mM 11-deoxycortisol, 0.5 mM PMSF or 20% glycerol Curvularia lunata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.45
-
 11-deoxycortisol 11beta-hydroxylation Curvularia lunata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 30 mM sodium phosphate buffer Curvularia lunata

Cofactor

Cofactor Comment Organism Structure
NADPH NADPH-linked cytochrome P-450 reductase Curvularia lunata