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Literature summary for 1.14.15.3 extracted from

  • van Beilen, J.B.; Smits, T.H.; Roos, F.F.; Brunner, T.; Balada, S.B.; Rothlisberger, M.; Witholt, B.
    Identification of an amino acid position that determines the substrate range of integral membrane alkane hydroxylases (2005), J. Bacteriol., 187, 85-91.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.3

Cloned(Commentary)

Cloned (Comment) Organism
-
 Alcanivorax borkumensis
expressed in Pseudomonas fluorescence KOB2delta1 Pseudomonas putida
expressed in Pseudomonas fluorescence KOB2delta1 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
H273A no activity Pseudomonas putida
H273Q no activity Pseudomonas putida
L69F oxidizes only C10 and C11 alkanes Mycobacterium tuberculosis
L69W oxidizes only C10 and C11 alkanes Mycobacterium tuberculosis
W55C prefers C11-C13 alkanes as growth substrates Pseudomonas putida
W55S prefers C11-C13 alkanes as growth substrates Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pseudomonas putida 16020
-
membrane
-
 Mycobacterium tuberculosis 16020
-
membrane
-
 Alcanivorax borkumensis 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
n-alkane + NAD(P)H + H+ + O2 Pseudomonas putida
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 Mycobacterium tuberculosis
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 Alcanivorax borkumensis
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 Pseudomonas putida GPo1 / ATCC29347
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 Mycobacterium tuberculosis H37Rv
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 Alcanivorax borkumensis AP1
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Alcanivorax borkumensis
-
-
-
Alcanivorax borkumensis AP1
-
-
-
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-
Pseudomonas putida
-
-
-
Pseudomonas putida GPo1 / ATCC29347
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
n-alkane + NAD(P)H + H+ + O2
-
 Pseudomonas putida n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Mycobacterium tuberculosis n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Alcanivorax borkumensis n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 native strain oxidizes C6-C13 alkanes Pseudomonas putida n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 oxidizes C10-C16 alkanes Mycobacterium tuberculosis n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Pseudomonas putida GPo1 / ATCC29347 n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 native strain oxidizes C6-C13 alkanes Pseudomonas putida GPo1 / ATCC29347 n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Mycobacterium tuberculosis H37Rv n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 oxidizes C10-C16 alkanes Mycobacterium tuberculosis H37Rv n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Alcanivorax borkumensis AP1 n-alkanol + NAD(P)+ + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
 Pseudomonas putida
NAD(P)H
-
 Mycobacterium tuberculosis
NAD(P)H
-
 Alcanivorax borkumensis