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Literature summary for 1.14.15.3 extracted from

  • Griffith, G.R.; Ruettinger, R.T.; McKenna, E.J.; Coon, M.J.
    Fatty acid omega-hydroxylase (alkane hydroxylase) from Pseudomonas oleovorans (1978), Methods Enzymol., 53, 356-360.
    View publication on PubMed
Search for more literature for 1.14.15.3

Inhibitors

Inhibitors Comment Organism Structure
KCN reversible inhibitor Pseudomonas oleovorans

Localization

Localization Comment Organism GeneOntology No. Textmining
cell membrane membrane-bound enzyme, needs 20 molecules phospholipid per polypeptide chain Pseudomonas oleovorans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 1 atom Fe per 42000 g/mol peptide Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
n-alkane + NAD(P)H + H+ + O2 Pseudomonas oleovorans
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
ferrous ions restore activity after treatment with EDTA and dithionite Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
-
 Pseudomonas oleovorans

Storage Stability

Storage Stability Organism
-20°C, 1 month, under 50% activity retained Pseudomonas oleovorans
-70°C, 12 days, over 90% activity retained Pseudomonas oleovorans
-70°C, 6 months, over 50% activity retained Pseudomonas oleovorans
4°C, 5 days, under 50% activity retained Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,7-octadiene + NADH + H+ + O2 epoxidation of simple, aliphatic terminal olefins Pseudomonas oleovorans 1,2-epoxy-7-octene + NAD+ + H2O
-
 ?
2,5-dimethylhexane + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
cyclohexane + NADH + H+ + O2
-
 Pseudomonas oleovorans cyclohexanol + NAD+ + H2O
-
 ?
fatty acid + NAD(P)H + H+ + O2 chain length C6-C14 Pseudomonas oleovorans omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
methylcyclohexane + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 rubredoxin is electron carrier Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 chain length C6-C14 Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay conditions Pseudomonas oleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay conditions Pseudomonas oleovorans

Cofactor

Cofactor Comment Organism Structure
NADH hydroxylation needs also a NADH-dependent bacterial reductase Pseudomonas oleovorans