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Literature summary for 1.14.15.3 extracted from

  • McKenna, E.J.; Coon, M.J.
    Enzymatic omega-oxidation. IV. Purification and properties of the omega-hydroxylase of Pseudomonas oleovorans (1970), J. Biol. Chem., 245, 3882-3889.
    View publication on PubMed
Search for more literature for 1.14.15.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 laurate
-
 Pseudomonas oleovorans
0.69
-
 Nonanoate
-
 Pseudomonas oleovorans
5.2
-
 Heptanoate
-
 Pseudomonas oleovorans
22
-
 hexanoate
-
 Pseudomonas oleovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe non-heme iron protein Pseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, SDS-PAGE Pseudomonas oleovorans
2000000
-
 gel filtration Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
n-alkane + NAD(P)H + H+ + O2 Pseudomonas oleovorans
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
596
-
-
 Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dimethylhexane + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
cyclohexane + NADH + H+ + O2
-
 Pseudomonas oleovorans cyclohexanol + NAD+ + H2O
-
 ?
fatty acid + NAD(P)H + H+ + O2 chain length C6-C11, maximal activity with heptanoate Pseudomonas oleovorans omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
heptanoate + NADPH + H+ + O2 59% activity compared to octane Pseudomonas oleovorans ?
-
 r
hexanoate + NADPH + H+ + O2 25% activity compared to octane Pseudomonas oleovorans ?
-
 r
lauric acid + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans 12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
 ?
lecithin + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
methylcyclohexane + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
monoolein + NADH + H+ + O2
-
 Pseudomonas oleovorans 18-hydroxyoctadec-9-enoic acid 2,3-dihydroxypropyl ester + NAD+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 rubredoxin is electron carrier Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 chain length C6-C16, maximal activity with n-octane Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
nonanoate + NADPH + H+ + O2 25% activity compared to octane Pseudomonas oleovorans ?
-
 r
phosphatidylethanolamine + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
phosphatidylserine + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Pseudomonas oleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 Tris buffer Pseudomonas oleovorans

pH Range

pH Minimum pH Maximum Comment Organism
6.7 7.5 laurate hydroxylation in Tris buffer Pseudomonas oleovorans

Cofactor

Cofactor Comment Organism Structure
NADH hydroxylation needs also a NADH-dependent bacterial reductase Pseudomonas oleovorans