Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
Q65A | site-directed mutagenesis, three-dimensional structure analysis and comparison to the wild-type enzyme | Mus musculus |
Q65E | site-directed mutagenesis, three-dimensional structure analysis and comparison to the wild-type enzyme | Mus musculus |
Q65H | site-directed mutagenesis, three-dimensional structure analysis and comparison to the wild-type enzyme | Mus musculus |
Q65L | site-directed mutagenesis, three-dimensional structure analysis and comparison to the wild-type enzyme | Mus musculus |
Q65N | site-directed mutagenesis, three-dimensional structure analysis and comparison to the wild-type enzyme | Mus musculus |
S408A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, three-dimensional structure analysis and comparison to the wild-type enzyme | Mus musculus |
S408I | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, three-dimensional structure analysis and comparison to the wild-type enzyme | Mus musculus |
S408T | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, three-dimensional structure analysis and comparison to the wild-type enzyme | Mus musculus |
S408V | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, three-dimensional structure analysis and comparison to the wild-type enzyme | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
25-hydroxyvitamin D | pH 7.4, 37°C, recombinant mutant S408T | Mus musculus | |
0.00018 | - |
25-hydroxyvitamin D | pH 7.4, 37°C, recombinant mutant S408I | Mus musculus | |
0.00024 | - |
25-hydroxyvitamin D | pH 7.4, 37°C, recombinant mutant S408A | Mus musculus | |
0.00028 | - |
25-hydroxyvitamin D | pH 7.4, 37°C, recombinant wild-type enzyme | Mus musculus | |
0.00052 | - |
1alpha-hydroxyvitamin D | pH 7.4, 37°C, recombinant wild-type enzyme | Mus musculus | |
0.00054 | - |
25-hydroxyvitamin D | pH 7.4, 37°C, recombinant mutant S408V | Mus musculus | |
0.00066 | - |
1alpha-hydroxyvitamin D | pH 7.4, 37°C, recombinant mutant S408V | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
25-hydroxyvitamin D + NADPH + O2 | Mus musculus | deficiency of the enzyme, due to mutation of residues Q65 and T409, in substrate binding causes vitamin D-dependent rickets type 1, overview | 1alpha,25-dihydroxyvitamin D + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1alpha-hydroxyvitamin D + NADPH + O2 | - |
Mus musculus | 1alpha,25-dihydroxyvitamin D + NADP+ + H2O | - |
? | |
25-hydroxyvitamin D + NADPH + O2 | deficiency of the enzyme, due to mutation of residues Q65 and T409, in substrate binding causes vitamin D-dependent rickets type 1, overview | Mus musculus | 1alpha,25-dihydroxyvitamin D + NADP+ + H2O | - |
? | |
25-hydroxyvitamin D + NADPH + O2 | binding of 25-hydroxyvitamin D3 in a structural pocket involving residues Q65, S408, and T409, molecular modeling and substrate docking | Mus musculus | 1alpha,25-dihydroxyvitamin D + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure analysis of wild-type and mutant enzymes, molecular modeling and substrate docking, overview | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
CYP27B1 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
1alpha-hydroxyvitamin D | pH 7.4, 37°C, recombinant mutant S408V | Mus musculus | |
0.0008 | - |
25-hydroxyvitamin D | pH 7.4, 37°C, recombinant mutant S408I | Mus musculus | |
0.006 | - |
25-hydroxyvitamin D | pH 7.4, 37°C, recombinant mutant S408A | Mus musculus | |
0.01 | - |
1alpha-hydroxyvitamin D | pH 7.4, 37°C, recombinant wild-type enzyme | Mus musculus | |
0.013 | - |
25-hydroxyvitamin D | pH 7.4, 37°C, recombinant mutant S408V | Mus musculus | |
0.098 | - |
25-hydroxyvitamin D | pH 7.4, 37°C, recombinant mutant S408T | Mus musculus | |
0.385 | - |
25-hydroxyvitamin D | pH 7.4, 37°C, recombinant wild-type enzyme | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mus musculus |