Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109, coexpression of adrenodoxin and NADPH-adrenodoxin reductase for reconstitution of the system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G125E | native mutant, no enzyme activity but normal expression level | Homo sapiens |
P382S | native mutant, no enzyme activity but normal expression level | Homo sapiens |
R107H | native mutant, no enzyme activity but normal expression level | Homo sapiens |
R335P | native mutant, no enzyme activity but normal expression level | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
(24R),25-dihydroxycholecalciferol | reconstituted system | Homo sapiens | |
0.0027 | - |
25-hydroxycholecalciferol | reconstituted system | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
25-hydroxycholecalciferol + NADPH + O2 | Homo sapiens | - |
calcitriol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000042 | - |
substrate 24-oxo-25-hydroxycholecalciferol, recombinant reconstituted system | Homo sapiens |
0.00000058 | - |
substrate 25-hydroxycholecalciferol, recombinant reconstituted system | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(24R),25-dihydroxycholecalciferol + NADPH + O2 | higher activity than with 25-hydroxycholecalciferol | Homo sapiens | 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O | - |
? | |
(24R),25-dihydroxycholecalciferol + NADPH + O2 | reconstituted system | Homo sapiens | 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O | - |
? | |
24-oxo-25-hydroxycholecalciferol + NADPH + O2 | low activity, reconstituted system | Homo sapiens | ? | - |
? | |
25-hydroxycholecalciferol + NADPH + O2 | - |
Homo sapiens | calcitriol + NADP+ + H2O | - |
? | |
calcidiol + NADPH + O2 | highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol | Homo sapiens | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? | |
additional information | not active with (23S),25-dihydroxycholecalciferol and 24-oxo-23,25-dihydroxycholecalciferol | Homo sapiens | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |