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Literature summary for 1.14.15.18 extracted from
- Purification and properties of vitamin D hydroxylases (1980), Methods Enzymol., 67, 430-440.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| malate | - |
Gallus gallus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Gallus gallus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11900 | - |
ferredoxin component, SDS-PAGE | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 25-hydroxycholecalciferol + NADPH + O2 | Gallus gallus | - |
calcitriol + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of ferredoxin component | Gallus gallus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | ferredoxin, ferredoxin reductase and cytochrome P-450 | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.758 | - |
purified ferredoxin, reduction of cytochrome c | Gallus gallus |
| 11.38 | - |
reconstituted system of cytochrome P-450, cytochrome b5, adrenocortical ferredoxin reductase, and renal ferredoxin | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 25-hydroxycholecalciferol + NADPH + O2 | - |
Gallus gallus | calcitriol + NADP+ + H2O | - |
? | |
| calcidiol + NADPH + O2 | highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol | Gallus gallus | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Gallus gallus | |
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