Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in Escherichia coli BL21(DE3) cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
I500F | the mutant shows quite a different metabolism of 1alpha,25-dihydroxyvitamin D3 from both human and rat CYP24A1 | Rattus norvegicus |
I500L | the mutant also shows the C-23 oxidation pathway | Rattus norvegicus |
I500T | the mutant also shows the C-23 oxidation pathway | Rattus norvegicus |
I500V | the mutant also shows the C-23 oxidation pathway | Rattus norvegicus |
T416A | the metabolic pattern by the mutant remains rat CYP24A1wild type | Rattus norvegicus |
T416F | the mutant has reaction specificity similar to human CYP24A1 by also showing the C-23 oxidation pathway | Rattus norvegicus |
T416I | the mutant has reaction specificity similar to human CYP24A1 by also showing the C-23 oxidation pathway | Rattus norvegicus |
T416M | the mutant also shows the C-23 oxidation pathway | Rattus norvegicus |
T416S | the metabolic pattern by the mutant remains rat CYP24A1wild type | Rattus norvegicus |
T416V | the mutant has reaction specificity similar to human CYP24A1 by also showing the C-23 oxidation pathway | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00014 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme I500T, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.00019 | - |
1alpha,25-dihydroxyvitamin D3 | wild type enzyme, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.00022 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416S, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.00024 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416A, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.0003 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme I500L, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.00035 | - |
1alpha,25-dihydroxyvitamin D3 | wild type enzyme, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.00066 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416M, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.00104 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416V, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.00114 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme I500F, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2 | the C-23 oxidation pathway | Homo sapiens | 1alpha,23S,25,26-tetrahydroxyvitamin D3 + 1alpha,23S,25-trihydroxyvitamin D3 + 25,26,27-trinor-23-ene-1alpha-hydroxyvitamin D3 + NADP+ + H2O | - |
? | |
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2 | the C-23 oxidation pathway, which is almost not shown by rat CYP24A1 | Rattus norvegicus | 1alpha,23S,25,26-tetrahydroxyvitamin D3 + 1alpha,23S,25-trihydroxyvitamin D3 + 25,26,27-trinor-23-ene-1alpha-hydroxyvitamin D3 + NADP+ + H2O | - |
? | |
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2 | the C-24 oxidation pathway | Homo sapiens | 24-oxo-1,23,25-trihydroxyvitamin D3 + 24,25,26,27-tetranor-1,23-dihydroxyvitamin D3 + 1alpha,24R,25-trihydroxyvitamin D3 + 24-oxo-1,25-dihydroxyvitamin D3 + NADP+ + H2O | - |
? | |
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2 | the C-24 oxidation pathway | Rattus norvegicus | 24-oxo-1,23,25-trihydroxyvitamin D3 + 24,25,26,27-tetranor-1,23-dihydroxyvitamin D3 + 1alpha,24R,25-trihydroxyvitamin D3 + 24-oxo-1,25-dihydroxyvitamin D3 + NADP+ + H2O | - |
? | |
additional information | human CYP24A1 catalyzes both C-23 and C-24 oxidation pathways of 1alpha,25-dihydroxyvitamin D3 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP24A1 | - |
Homo sapiens |
CYP24A1 | - |
Rattus norvegicus |
vitamin D 24-hydroxylase | - |
Homo sapiens |
vitamin D 24-hydroxylase | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme I500L, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.002 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme I500F, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.002 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme I500T, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.0035 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416M, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.004 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416A, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.004 | - |
1alpha,25-dihydroxyvitamin D3 | wild type enzyme, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.005 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416S, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.007 | - |
1alpha,25-dihydroxyvitamin D3 | wild type enzyme, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.012 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416V, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens | |
NADPH | - |
Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme I500F, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
2.8 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme I500L, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
5.3 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416M, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
11.8 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416V, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
15.3 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme I500T, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
20 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416A, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
20 | - |
1alpha,25-dihydroxyvitamin D3 | wild type enzyme, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Homo sapiens | |
23.3 | - |
1alpha,25-dihydroxyvitamin D3 | mutant enzyme T416S, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
23.3 | - |
1alpha,25-dihydroxyvitamin D3 | wild type enzyme, in 100 mM Tris-HCl, pH 7.4, temperature not specified in the publication | Rattus norvegicus |