Activating Compound | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | required for activity of the recombinant enzyme | Homo sapiens | |
adrenodoxin reductase | required for activity of the recombinant enzyme | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Sf21 insect cells using the previously cloned cDNA in baculovirus (AcNPV-P450cc24), solubilized mitochondrial protein from Sf21 cells infected with recombinant virus AcNPV-P450cc24 cDNA express 24-hydroxylase activity when reconstituted with bovine adrenodoxin, adrenodoxin reductase, and NADPH | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
25-hydroxyvitamin D3 + NADPH + H+ + O2 | - |
Homo sapiens | 24-oxo-25-hydroxyvitamin D3 + 23(S),25-dihydroxyvitamin D3 + 24(R),25-dihydroxyvitamin D3 + 24-oxo-23(S),25-dihydoxyvitamin D3 + NADP+ + H2O | - |
? | |
25-hydroxyvitamin D3 + NADPH + H+ + O2 | - |
Homo sapiens | 24,25,26,27-tetranor-23-hydroxyvitamin-D3 + NADP+ + H2O | - |
? | |
calcidiol + NADPH + H+ + O2 | - |
Homo sapiens | secalciferol + NADP+ + H2O | - |
? | |
calcitriol + NADPH + H+ + O2 | the h-P450cc24 activity is approximately 10fold higher for calcitriol than for calcidiol | Homo sapiens | calcitetrol + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
25-hydroxyvitamin D3-24-hydroxylase | - |
Homo sapiens |
P450cc24 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | required for activity of the recombinant enzyme | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | P450cc24 is a multicatalytic enzyme catalyzing most, if not all, of the reactions in the C-24/C-23 pathway of 25-hydroxyvitamin D3 (calcidiol) metabolism | Homo sapiens |