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Literature summary for 1.14.15.15 extracted from
- Multi-functional property of rat liver mitochondrial cytochrome P-450 (1991), J. Biochem., 109, 389-393.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1alpha-hydroxyvitamin D3 | competes with 5beta-cholestane-3alpha,7alpha,12alpha-triol | Rattus norvegicus |  |
| 5beta-cholestane-3alpha,7alpha,12alpha-triol | competes with 1alpha-hydroxyvitamin D3 | Rattus norvegicus | |
| N-bromosuccinimide | 50% loss of activity at 0.1 mM | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00233 | - |
1alpha-hydroxyvitamin D3 as substrate | Rattus norvegicus |
| 0.114 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol as substrate | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1alpha-hydroxyvitamin D3 + reduced adrenodoxin + O2 | 25-hydroxylation | Rattus norvegicus | 1alpha,25-dihydroxyvitamin D3 + oxidized adrenodoxin + H2O | - |
? | |
| 5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | 5beta-cholestane-3alpha,7alpha,12alpha,27-tetrol + oxidized adrenodoxin + H2O | - |
? | |
| 5beta-cholestane-3alpha,7alpha-diol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
90% loss of activity after 2 min | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0233 | - |
1alpha-hydroxyvitamin D3 | - |
Rattus norvegicus | |
| 0.217 | - |
5beta-cholestane-3alpha,7alpha-diol | - |
Rattus norvegicus | |
| 0.6 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol | - |
Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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