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Literature summary for 1.14.15.1 extracted from
- A functional proline switch in cytochrome P450cam (2008), Structure, 16, 916-923.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | involvement of X-proline isomerization in enzyme function | Pseudomonas putida |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutants overexpressed from Escherichia coli strain NCM533 harboring modified pDNC334A plasmids that encode the appropriate mutant of CYP101 under control of the lac promoter | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C334A | mutant, that is spectroscopically and enzymatically identical to wild-type CYP101 enzyme, but does not form dimers in solution, and so is more suitable for solution NMR studies than wild-type enzyme | Pseudomonas putida |
| P89I | yields a mixture of both bound camphor orientations, that seen in putidaredoxin-free and that seen in putidaredoxin-bound CYP101. A mutation in CYP101 that destabilizes the cis conformer of the Ile-88-Pro-89 amide bond results in weaker binding of putidaredoxin | Pseudomonas putida |
| Y29F | the cis conformer is destabilized by the absence of the hydrogen bond between the carbonyl oxygen of Ile-88 and the Tyr-29 hydroxyl group | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-camphor + O2 + reduced putidaredoxin | conformational change in CYP101 upon binding of putidaredoxin that re-orients bound camphor appropriately for hydroxylation | Pseudomonas putida | exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP101 | - |
Pseudomonas putida |
| cytochrome p450cam | - |
Pseudomonas putida |
| P450cam | - |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| putidaredoxin | binding of putidaredoxin converts a single X-proline amide bond in CYP101 from trans or distorted trans to cis | Pseudomonas putida |
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