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Literature summary for 1.14.15.1 extracted from

  • Gould, P.V.; Gelb, M.H.; Sligar, S.G.
    Interaction of 5-bromocamphor with cytochrome P-450 cam. Production of 5-ketocamphor from a mixed spin state hemoprotein (1981), J. Biol. Chem., 256, 6686-6691.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Pseudomonas putida 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ requirement, enzyme complex with two FeS-protein components Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + putidaredoxin + O2 Pseudomonas putida
-
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
PpG786, mutant derived from PpG1
-
Pseudomonas putida
-
Accession Number PC00183
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + putidaredoxin + O2
-
Pseudomonas putida (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
5-exo-bromocamphor + putidaredoxin + O2 (+)- and (-)-enantiomer Pseudomonas putida 5-ketocamphor + oxidized putidaredoxin + Br- + H2O
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
cytochrome m
-
Pseudomonas putida
NADH
-
Pseudomonas putida
putidaredoxin essential, iron-sulfur redox protein, Fe2S2*Cys4-class, e-transfer agent to and effector of cytochrome P450cam Pseudomonas putida