Literature summary for 1.14.14.9 extracted from

Coulombel, L.; Nolan, L.C.; Nikodinovic, J.; Doyle, E.M.; OConnor, K.E.
Biotransformation of 4-halophenols to 4-halocatechols using Escherichia coli expressing 4-hydroxyphenylacetate 3-hydroxylase (2011), Appl. Microbiol. Biotechnol., 89, 1867-1875.

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Application

Application	Comment	Organism
synthesis	enzyme completely transforms 4-substituted halophenols to 4-halocatechols at 2 mM within a 1-2 h period. An increase in 4-halophenol concentration to 4.8 mM results in a 2.5-20fold decrease in biotransformation efficiency depending on the substrate tested. Organic solvent extraction of the 4-halocatechol products followed by column chromatography results in the production of purified products with a final yield of between 33% and 38%	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	Q57160	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-bromophenol + NADH + H+ + O2	rate of halophenol consumption 2.9 microM/min/g cell dry weight	Escherichia coli	4-bromocatechol + NAD+ + H2O	-	?
4-chlorophenol + NADH + H+ + O2	rate of halophenol consumption 3.3 microM/min/g cell dry weight	Escherichia coli	4-chlorocatechol + NAD+ + H2O	-	?
4-fluorophenol + NADH + H+ + O2	rate of halophenol consumption 5.3 microM/min/g cell dry weight	Escherichia coli	4-fluorocatechol + NAD+ + H2O	-	?
4-iodophenol + NADH + H+ + O2	rate of halophenol consumption 1.7 microM/min/g cell dry weight	Escherichia coli	4-iodocatechol + NAD+ + H2O	-	?

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Release 2023.1 (January 2023)