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Literature summary for 1.14.14.9 extracted from

  • Raju, S.G.; Kamath, A.V.; Vaidyanathan, C.S.
    Purification and properties of 4-hydroxyphenylacetic acid 3-hydroxylase from Pseudomonas putida (1988), Biochem. Biophys. Res. Commun., 154, 537-543.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Carbohydrates stimulate synthesis of enzyme insignificantly Pseudomonas putida
additional information not activated by beta-mercaptoethanol, glutathione and dithiothreiotol Pseudomonas putida

General Stability

General Stability Organism
unstable Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
Cibacron blue F3GA dye Pseudomonas putida
Cu2+
-
Pseudomonas putida
Hg2+
-
Pseudomonas putida
additional information not inhibited by Mn2+, Mg2+, Fe3+ and Fe2+ Pseudomonas putida
N-ethylmaleimide 48% inhibition at 1 mM Pseudomonas putida
p-chloromercuribenzoate 92% inhibition at 1 mM Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.059
-
NADH
-
Pseudomonas putida
0.2
-
4-hydroxyphenylacetate
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxyphenylacetate + NADH + O2 Pseudomonas putida key enzyme in pathway of degradation of phenylalanine, tyrosine and other aromatic amines 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, gel filtration, affinity chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
maximal in enzymes of the late log phase Pseudomonas putida
6.5
-
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
4°C, 15-20 days Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyphenylacetate + NADH + O2
-
Pseudomonas putida 3,4-hydroxyphenylacetate + NAD+ + H2O
-
?
4-fluorophenylacetate + NADH + O2
-
Pseudomonas putida 4-fluoro-3-hydroxyphenylacetate + NAD+ + H2O
-
?
4-hydroxyphenylacetate + NADH + O2
-
Pseudomonas putida 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?
4-hydroxyphenylacetate + NADH + O2 key enzyme in pathway of degradation of phenylalanine, tyrosine and other aromatic amines Pseudomonas putida 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * x, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas putida
45
-
-
Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
15-20 days Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein monooxygenase, absolute requirement Pseudomonas putida
NADH absolute requirement Pseudomonas putida