Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Carbohydrates | stimulate synthesis of enzyme insignificantly | Pseudomonas putida | |
additional information | not activated by beta-mercaptoethanol, glutathione and dithiothreiotol | Pseudomonas putida |
General Stability | Organism |
---|---|
unstable | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cibacron blue F3GA | dye | Pseudomonas putida | |
Cu2+ | - |
Pseudomonas putida | |
Hg2+ | - |
Pseudomonas putida | |
additional information | not inhibited by Mn2+, Mg2+, Fe3+ and Fe2+ | Pseudomonas putida | |
N-ethylmaleimide | 48% inhibition at 1 mM | Pseudomonas putida | |
p-chloromercuribenzoate | 92% inhibition at 1 mM | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
NADH | - |
Pseudomonas putida | |
0.2 | - |
4-hydroxyphenylacetate | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + O2 | Pseudomonas putida | key enzyme in pathway of degradation of phenylalanine, tyrosine and other aromatic amines | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, gel filtration, affinity chromatography | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
maximal in enzymes of the late log phase | Pseudomonas putida |
6.5 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C, 15-20 days | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyphenylacetate + NADH + O2 | - |
Pseudomonas putida | 3,4-hydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-fluorophenylacetate + NADH + O2 | - |
Pseudomonas putida | 4-fluoro-3-hydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | - |
Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | key enzyme in pathway of degradation of phenylalanine, tyrosine and other aromatic amines | Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * x, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
45 | - |
- |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
15-20 days | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein monooxygenase, absolute requirement | Pseudomonas putida | |
NADH | absolute requirement | Pseudomonas putida |