Protein Variants | Comment | Organism |
---|---|---|
A75G | site-directed mutagenesis, activity similar to the wild-type enzyme | Vibrio harveyi |
C106V | site-directed mutagenesis, highly reduced ability to stabilize the reaction intermediate due to interaction between Val106 and Ala75 side chains, and therefore highly reduced activity and increased thermal lability compared to the wild-type enzyme | Vibrio harveyi |
C106V/A75G | site-directed mutagenesis, mutation of Ala75 restores about 90% of the activity abolished by mutation of Cys106, shift in the light emission spectrum to that of Photobacterium phosphoreum possessing Val and Gly at positions 106 and 75, respectively | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, substrate and cofactor binding | Vibrio harveyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
RCHO + FMNH2 + O2 | Vibrio harveyi | - |
RCOOH + FMN + H2O + hn | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a long-chain aldehyde + FMNH2 + O2 = a long-chain fatty acid + FMN + H2O + hnu | reaction mechanism, Cys106 is essential for stabilization of the reaction intermediate, for consumption of aldehyde substrate, and for activity | Vibrio harveyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
decanal + FMNH2 + O2 | - |
Vibrio harveyi | decanoate + FMN + H2O + hn | - |
ir | |
dodecanal + FMNH2 + O2 | - |
Vibrio harveyi | dodecanoate + FMN + H2O + hn | - |
ir | |
nonanal + FMNH2 + O2 | - |
Vibrio harveyi | nonanoate + FMN + H2O + hn | - |
ir | |
octanal + FMNH2 + O2 | - |
Vibrio harveyi | octanoate + FMN + H2O + hn | - |
ir | |
RCHO + FMNH2 + O2 | - |
Vibrio harveyi | RCOOH + FMN + H2O + hn | - |
ir | |
RCHO + FMNH2 + O2 | formation of a C4a-hydroperoxyflavin intermediate | Vibrio harveyi | RCOOH + FMN + H2O + hn | - |
ir | |
undecanal + FMNH2 + O2 | - |
Vibrio harveyi | undecanoate + FMN + H2O + hn | - |
ir |
Subunits | Comment | Organism |
---|---|---|
More | structure-function relationship, roles of Cys106, Ala75, Ala74, and the isoalloxazine ring of FMN | Vibrio harveyi |
Synonyms | Comment | Organism |
---|---|---|
luciferase | - |
Vibrio harveyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Vibrio harveyi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
wild-type enzyme and mutanbt A75G: loss of 60% activity within 50 min, mutants C106V and C106V/A75G show increased thermolability loosing 99% and 90% activity, respectively | Vibrio harveyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | single turnover, luminescence | Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Vibrio harveyi |