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Literature summary for 1.14.14.3 extracted from

  • Szittner, R.; Meighen, E.
    Nucleotide sequence, expression, and properties of luciferase coded by lux genes from a terrestrial bacterium (1990), J. Biol. Chem., 265, 16581-16587.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Vibrio harveyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000 78000
-
Vibrio harveyi
77000 78000 gel filtration Photorhabdus luminescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
decanal + FMNH + O2 Vibrio harveyi
-
decanoic acid + FMN + H2O + light
-
ir
decanal + FMNH + O2 Photorhabdus luminescens
-
decanoic acid + FMN + H2O + light
-
ir

Organism

Organism UniProt Comment Textmining
Photorhabdus luminescens
-
-
-
Vibrio harveyi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
decanal + FMNH + O2
-
Vibrio harveyi decanoic acid + FMN + H2O + light
-
ir
decanal + FMNH + O2
-
Photorhabdus luminescens decanoic acid + FMN + H2O + light
-
ir
dodecanal + FMNH + O2
-
Vibrio harveyi dodecanoic acid + FMN + H2O + light
-
ir
dodecanal + FMNH + O2
-
Photorhabdus luminescens dodecanoic acid + FMN + H2O + light
-
ir
octanal + FMNH + O2
-
Vibrio harveyi octanoic acid + FMN + H2O + light
-
ir
octanal + FMNH + O2
-
Photorhabdus luminescens octanoic acid + FMN + H2O + light
-
ir

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
half-life over 3 h Photorhabdus luminescens
45
-
half-life 5 min Vibrio harveyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Vibrio harveyi
additional information
-
additional information tetradecanal + FMNH + O2 (possibly) turnover rate determined by measuring the half-time for decay of luminescence between 70% and 35% of the maximum intensity, values depending on chain-length of aldehyde Photorhabdus luminescens