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Literature summary for 1.14.14.20 extracted from

  • Duffner, F.M.; Kirchner, U.; Bauer, M.P.; Mueller, R.
    Phenol/cresol degradation by the thermophilic Bacillus thermoglucosidasius A7: cloning and sequence analysis of five genes involved in the pathway (2000), Gene, 256, 215-221.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pheA1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli, coexpression with pheA2 is required for catalytic activity Parageobacillus thermoglucosidasius
gene pheA2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli, coexpression with pheA1 is required for catalytic activity Parageobacillus thermoglucosidasius

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Parageobacillus thermoglucosidasius 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
x * 18000, about, sequence calculation Parageobacillus thermoglucosidasius
57000
-
x * 57000, about, sequence calculation Parageobacillus thermoglucosidasius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenol + FADH2 + O2 Parageobacillus thermoglucosidasius
-
catechol + FAD + H2O
-
?
phenol + FADH2 + O2 Parageobacillus thermoglucosidasius A7
-
catechol + FAD + H2O
-
?

Organism

Organism UniProt Comment Textmining
Parageobacillus thermoglucosidasius Q9LAG2 PheA2; or Bacillus thermoglucosidasius, gene pheA2
-
Parageobacillus thermoglucosidasius Q9LAG3 PheA1; or Bacillus thermoglucosidasius, gene pheA1
-
Parageobacillus thermoglucosidasius A7 Q9LAG2 PheA2; or Bacillus thermoglucosidasius, gene pheA2
-
Parageobacillus thermoglucosidasius A7 Q9LAG3 PheA1; or Bacillus thermoglucosidasius, gene pheA1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenol + FADH2 + O2
-
Parageobacillus thermoglucosidasius catechol + FAD + H2O
-
?
phenol + FADH2 + O2
-
Parageobacillus thermoglucosidasius A7 catechol + FAD + H2O
-
?

Subunits

Subunits Comment Organism
? x * 18000, about, sequence calculation Parageobacillus thermoglucosidasius
? x * 57000, about, sequence calculation Parageobacillus thermoglucosidasius

Cofactor

Cofactor Comment Organism Structure
FADH2
-
Parageobacillus thermoglucosidasius

General Information

General Information Comment Organism
physiological function Geobacillus thermoglucosidasius strain A7 degrades phenol at 65°C via the meta cleavage pathway Parageobacillus thermoglucosidasius