Cloned (Comment) | Organism |
---|---|
gene pheA1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli, coexpression with pheA2 is required for catalytic activity | Parageobacillus thermoglucosidasius |
gene pheA2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli, coexpression with pheA1 is required for catalytic activity | Parageobacillus thermoglucosidasius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Parageobacillus thermoglucosidasius | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 18000, about, sequence calculation | Parageobacillus thermoglucosidasius |
57000 | - |
x * 57000, about, sequence calculation | Parageobacillus thermoglucosidasius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenol + FADH2 + O2 | Parageobacillus thermoglucosidasius | - |
catechol + FAD + H2O | - |
? | |
phenol + FADH2 + O2 | Parageobacillus thermoglucosidasius A7 | - |
catechol + FAD + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Parageobacillus thermoglucosidasius | Q9LAG2 | PheA2; or Bacillus thermoglucosidasius, gene pheA2 | - |
Parageobacillus thermoglucosidasius | Q9LAG3 | PheA1; or Bacillus thermoglucosidasius, gene pheA1 | - |
Parageobacillus thermoglucosidasius A7 | Q9LAG2 | PheA2; or Bacillus thermoglucosidasius, gene pheA2 | - |
Parageobacillus thermoglucosidasius A7 | Q9LAG3 | PheA1; or Bacillus thermoglucosidasius, gene pheA1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenol + FADH2 + O2 | - |
Parageobacillus thermoglucosidasius | catechol + FAD + H2O | - |
? | |
phenol + FADH2 + O2 | - |
Parageobacillus thermoglucosidasius A7 | catechol + FAD + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 18000, about, sequence calculation | Parageobacillus thermoglucosidasius |
? | x * 57000, about, sequence calculation | Parageobacillus thermoglucosidasius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | - |
Parageobacillus thermoglucosidasius |
General Information | Comment | Organism |
---|---|---|
physiological function | Geobacillus thermoglucosidasius strain A7 degrades phenol at 65°C via the meta cleavage pathway | Parageobacillus thermoglucosidasius |