Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in yeast microsomes, expression of modified CYP17A1 in Escherichia coli strain JM109 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A105L | site-directed mutagenesis, the mutant shows low 21-hydroxylation activity compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | calculation of intramolecular and intermolecular kinetic isotope effects for wild-type and mutant enzymes, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
17alpha-hydroxyprogesterone + 16alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P05093 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from yeast mcirosomes, recombinant modified CYP17A1 from Escherichia coli strain JM109 to homogeneity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | human CYP17A1 also has progesterone 21-hydroxylase activity, EC 1.14.99.10. Product analysis by LC-MS/MS | Homo sapiens | ? | - |
? | |
pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 17alpha-hydroxyprogesterone + 16alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
17-hydroxylase/17,20-lyase | - |
Homo sapiens |
CYP17A1 | - |
Homo sapiens |
P450c17 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |