Literature summary for 1.14.14.19 extracted from

Imai, T.; Globerman, H.; Gertner, J.M.; Kagawa, N.; Waterman, M.R.
Expression and purification of functional human 17alpha-hydroxylase/17,20-lyase (P450c17) in Escherichia coli. Use of this system for study of a novel form of combined 17alpha-hydroxylase/17,20-lyase deficiency (1993), J. Biol. Chem., 268, 19681-19689.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, human gene with an (His)4-tail	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0017	-	17alpha-hydroxypregnenolone	-	Homo sapiens
0.0063	-	progesterone	-	Homo sapiens
0.0077	-	pregnenolone	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pregnenolone + AH2 + O2	Homo sapiens	-	17alpha-hydroxypregnenolone + A + H2O	product eliminates at C20,21 acetate to yield dehydroepiandrosterone	?
progesterone + [reduced NADPH-hemoprotein reductase] + O2	Homo sapiens	-	17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O	product eliminates at C20,21 acetate to yield androstenedione	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pregnenolone + AH2 + O2	-	Homo sapiens	17alpha-hydroxypregnenolone + A + H2O	product eliminates at C20,21 acetate to yield dehydroepiandrosterone	?
progesterone + [reduced NADPH-hemoprotein reductase] + O2	-	Homo sapiens	17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O	product eliminates at C20,21 acetate to yield androstenedione	?

Synonyms

Synonyms	Comment	Organism
P450c17	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00283	-	17alpha-hydroxypregnenolone	-	Homo sapiens
0.0483	-	progesterone	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Homo sapiens

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Release 2023.1 (January 2023)