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Literature summary for 1.14.14.19 extracted from
- Molecular modeling of human P450c17 (17alpha-hydroxylase/17,20-lyase): insights into reaction mechanisms and effects of mutations (1999), Mol. Endocrinol., 13, 1169-1182.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 | acts as allosteric facilitator but not as electron donor, increases 17,20-lyase activity | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisiae strain W303B of the K89N-mutant | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K89N | 78% loss of 17,20-lyase activity and 20% loss of 17alpha-hydroxylase activity | Homo sapiens |
| R347H | loss of 17,20-lyase activity to a greater extent than 17alpha-hydroxylase activity | Homo sapiens |
| R358Q | loss of 17,20-lyase activity to a greater extent than 17alpha-hydroxylase activity | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 17-hydroxypregnenolone | competitive inhibitor of 17alpha-hydrolase activity | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
17-hydroxypregnenolone | wild type enzyme | Homo sapiens | |
| 0.0008 | - |
17-hydroxypregnenolone | K89N mutant expressed in Saccharomyces cerevisiae | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | heme | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | it catalyzes steroid 17alpha-hydroxylase and 17,20-lyase activities in the biosynthesis of androgens, estrogens and cortisol | ? | - |
? | |
| pregnenolone + AH2 + O2 | Homo sapiens | - |
17alpha-hydroxypregnenolone + A + H2O | product eliminates at C20,21 acetate to yield dehydroepiandrosterone | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P05093 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | both the lyase and hydroxylase activities proceed from a common steroid-binding geometry by an iron oxene mechanism | Homo sapiens | ? | - |
? | |
| additional information | it catalyzes steroid 17alpha-hydroxylase and 17,20-lyase activities in the biosynthesis of androgens, estrogens and cortisol | Homo sapiens | ? | - |
? | |
| pregnenolone + AH2 + O2 | - |
Homo sapiens | 17alpha-hydroxypregnenolone + A + H2O | product eliminates at C20,21 acetate to yield dehydroepiandrosterone | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| P450c17 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
17-hydroxypregnenolone | R347H-mutant, inhibiton of 17alpha-hydrolase activity | Homo sapiens | |
| 0.0005 | - |
17-hydroxypregnenolone | K89N-mutant, inhibiton of 17alpha-hydrolase activity | Homo sapiens | |
| 0.0007 | - |
17-hydroxypregnenolone | wild type, inhibiton of 17alpha-hydrolase activity | Homo sapiens | |
| 0.0008 | - |
17-hydroxypregnenolone | R358Q-mutant, inhibiton of 17alpha-hydrolase activity | Homo sapiens | |
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