Protein Variants | Comment | Organism |
---|---|---|
H26A/K34A/K132A | mutant does not interact with holo-PhuS and shows no enzymatic activity | Pseudomonas aeruginosa |
N19K/K34A/F117Y/K132A | change in regioselectivity, product is biliverdin IXalpha | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | O69002 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoheme + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pseudomonas aeruginosa | biliverdin IXbeta + biliverdin IXdelta + Fe2+ + CO + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
biliverdin-producing heme oxygenase | - |
Pseudomonas aeruginosa |
PigA | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54 | 56 | melting temperature, wild-type and mutants H26A/K34A/K132A and N19K/K34A/F117Y/K132A | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | sprectrum shows a Soret band at 407 nm | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme interacts and acquires heme from cytoplasmic heme transport protein holo-PhuS. In mutant strains, the absence of bilverdin IXbeta and bilverdinIXdelta leads to a decrease in extracellular levels of hemophore HasA | Pseudomonas aeruginosa |