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Literature summary for 1.14.14.18 extracted from
- An improved method for purification of recombinant truncated heme oxygenase-1 by expanded bed adsorption and gel filtration (2007), Bioprocess Biosyst. Eng., 30, 87-90.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH 5a cells | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-ion exchange expanded bed adsorption and Superdex 75 gel filtration, the specific HO-1 activity increases from 0.82 to 24.8 U/mg during purification steps | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the specific HO-1 activity increases from 0.82 to 24.8 U/mg during purification steps | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| heme oxygenase-1 | - |
Homo sapiens |
| HO-1 | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | binding capacity increases with the increasing pH from 7 to 9 | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | truncated HO-1 does not bind on the adsorbent at low pH 6 and 6.5 | Homo sapiens |
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