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Literature summary for 1.14.14.18 extracted from
- Purification and properties of bovine spleen heme oxygenase. Amino acid composition and sites of action of inhibitors of heme oxidation (1982), J. Biol. Chem., 257, 7778-7785.
General Stability
| General Stability | Organism |
|---|---|
| 10-20% loss of activity upon thawing | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co-protoporphyrin | - |
Bos taurus |  |
| Hg2+ | inhibition of NADPH-cytochrome c reductase or biliverdin reductase in reconstituted heme oxygenase system | Bos taurus | |
| Mn-protoporphyrin | - |
Bos taurus | |
| p-chloromercuribenzoate | inhibition of NADPH-cytochrome c reductase in reconstituted heme oxygenase system | Bos taurus | |
| Porphyrins | metalloporphyrins | Bos taurus | |
| Sn-protoporphyrin | - |
Bos taurus | |
| Zn-protoporphyrin | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00093 | - |
heme | - |
Bos taurus | |
| 0.023 | - |
NADPH | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31000, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Triton X-100 + cholate, DEAE-cellulose, hydroxyapatite, Sepharose CL-6B, hydroxyapatite | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.113 | - |
spleen enzyme | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 20 mM potassium phosphate, pH 7.5, 0.2% Triton X-100, 20% glycerol, 2 d, 50% loss of activity | Bos taurus |
| liquid nitrogen, 6 months, no loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| heme + 2 NADPH + 2 H+ + 2 O2 | - |
Bos taurus | biliverdin + Fe2+ + CO + 2 NADP+ + H2O | - |
? | |
| heme + electron donor + O2 | NADH can replace NADPH at concentrations higher than 5 mM in vitro, NADH is unlikely to be an electron donor in vivo | Bos taurus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
| heme + electron donor + O2 | electron donor NADPH | Bos taurus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0583 | - |
heme | - |
Bos taurus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000033 | - |
Sn-protoporphyrin | - |
Bos taurus | |
| 0.000082 | - |
Co-protoporphyrin | - |
Bos taurus | |
| 0.00013 | - |
Zn-protoporphyrin | - |
Bos taurus | |
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