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Literature summary for 1.14.14.18 extracted from

  • Schacter, B.A.
    Assay of microsomal heme oxygenase in liver and spleen (1978), Methods Enzymol., 52, 367-372.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline weak Bos taurus
1,10-phenanthroline weak Gallus gallus
1,10-phenanthroline weak Homo sapiens
1,10-phenanthroline weak Oryctolagus cuniculus
1,10-phenanthroline weak Rattus norvegicus
1,10-phenanthroline weak Sus scrofa
2,2'-dipyridyl weak Bos taurus
2,2'-dipyridyl weak Gallus gallus
2,2'-dipyridyl weak Homo sapiens
2,2'-dipyridyl weak Oryctolagus cuniculus
2,2'-dipyridyl weak Rattus norvegicus
2,2'-dipyridyl weak Sus scrofa
4-hydroxymercuribenzoate
-
Bos taurus
4-hydroxymercuribenzoate
-
Gallus gallus
4-hydroxymercuribenzoate
-
Homo sapiens
4-hydroxymercuribenzoate
-
Oryctolagus cuniculus
4-hydroxymercuribenzoate
-
Rattus norvegicus
4-hydroxymercuribenzoate
-
Sus scrofa
CO strong inhibition Bos taurus
CO strong inhibition Gallus gallus
CO strong inhibition Homo sapiens
CO strong inhibition Oryctolagus cuniculus
CO strong inhibition Rattus norvegicus
CO strong inhibition Sus scrofa
Co2+ 0.4 mM, significant inhibition Bos taurus
Co2+ 0.4 mM, significant inhibition Gallus gallus
Co2+ 0.4 mM, significant inhibition Homo sapiens
Co2+ 0.4 mM, significant inhibition Oryctolagus cuniculus
Co2+ 0.4 mM, significant inhibition Rattus norvegicus
Co2+ 0.4 mM, significant inhibition Sus scrofa
Cu2+ 0.2 mM, significant inhibition Bos taurus
Cu2+ 0.2 mM, significant inhibition Gallus gallus
Cu2+ 0.2 mM, significant inhibition Homo sapiens
Cu2+ 0.2 mM, significant inhibition Oryctolagus cuniculus
Cu2+ 0.2 mM, significant inhibition Rattus norvegicus
Cu2+ 0.2 mM, significant inhibition Sus scrofa
cysteine
-
Bos taurus
cysteine
-
Gallus gallus
cysteine
-
Homo sapiens
cysteine
-
Oryctolagus cuniculus
cysteine
-
Rattus norvegicus
cysteine
-
Sus scrofa
EDTA weak Bos taurus
EDTA weak Gallus gallus
EDTA weak Homo sapiens
EDTA weak Oryctolagus cuniculus
EDTA weak Rattus norvegicus
EDTA weak Sus scrofa
KCN strong inhibition Bos taurus
KCN strong inhibition Gallus gallus
KCN strong inhibition Homo sapiens
KCN strong inhibition Oryctolagus cuniculus
KCN strong inhibition Rattus norvegicus
KCN strong inhibition Sus scrofa
NaN3 strong inhibition Bos taurus
NaN3 strong inhibition Gallus gallus
NaN3 strong inhibition Homo sapiens
NaN3 strong inhibition Oryctolagus cuniculus
NaN3 strong inhibition Rattus norvegicus
NaN3 strong inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
protoheme IX enzyme from spleen and liver Rattus norvegicus
0.0164
-
protoheme IX enzyme from spleen Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Gallus gallus
-
-
microsome
-
Homo sapiens
-
-
microsome
-
Rattus norvegicus
-
-
microsome
-
Sus scrofa
-
-
microsome
-
Bos taurus
-
-
microsome
-
Oryctolagus cuniculus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
gel filtration Rattus norvegicus
200000
-
gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
heme + 2 NADPH + 2 H+ + 2 O2 Gallus gallus involved in heme metabolism biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?
heme + 2 NADPH + 2 H+ + 2 O2 Homo sapiens involved in heme metabolism biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?
heme + 2 NADPH + 2 H+ + 2 O2 Rattus norvegicus involved in heme metabolism biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?
heme + 2 NADPH + 2 H+ + 2 O2 Sus scrofa involved in heme metabolism biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?
heme + 2 NADPH + 2 H+ + 2 O2 Bos taurus involved in heme metabolism biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?
heme + 2 NADPH + 2 H+ + 2 O2 Oryctolagus cuniculus involved in heme metabolism biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Gallus gallus
-
-
-
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Gallus gallus
-
brain
-
Homo sapiens
-
brain
-
Rattus norvegicus
-
brain
-
Sus scrofa
-
brain
-
Bos taurus
-
brain
-
Oryctolagus cuniculus
-
heart
-
Gallus gallus
-
heart
-
Homo sapiens
-
heart
-
Rattus norvegicus
-
heart
-
Sus scrofa
-
heart
-
Bos taurus
-
heart
-
Oryctolagus cuniculus
-
kidney
-
Gallus gallus
-
kidney
-
Homo sapiens
-
kidney
-
Rattus norvegicus
-
kidney
-
Sus scrofa
-
kidney
-
Bos taurus
-
kidney
-
Oryctolagus cuniculus
-
liver
-
Gallus gallus
-
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-
liver
-
Bos taurus
-
liver
-
Oryctolagus cuniculus
-
lung
-
Gallus gallus
-
lung
-
Homo sapiens
-
lung
-
Rattus norvegicus
-
lung
-
Sus scrofa
-
lung
-
Bos taurus
-
lung
-
Oryctolagus cuniculus
-
macrophage peritoneal, alveolar Gallus gallus
-
macrophage peritoneal, alveolar Homo sapiens
-
macrophage peritoneal, alveolar Rattus norvegicus
-
macrophage peritoneal, alveolar Sus scrofa
-
macrophage peritoneal, alveolar Bos taurus
-
macrophage peritoneal, alveolar Oryctolagus cuniculus
-
marrow
-
Gallus gallus
-
marrow
-
Homo sapiens
-
marrow
-
Rattus norvegicus
-
marrow
-
Sus scrofa
-
marrow
-
Bos taurus
-
marrow
-
Oryctolagus cuniculus
-
small intestine mucosa
-
Gallus gallus
-
small intestine mucosa
-
Homo sapiens
-
small intestine mucosa
-
Rattus norvegicus
-
small intestine mucosa
-
Sus scrofa
-
small intestine mucosa
-
Bos taurus
-
small intestine mucosa
-
Oryctolagus cuniculus
-
spleen
-
Gallus gallus
-
spleen
-
Homo sapiens
-
spleen
-
Rattus norvegicus
-
spleen
-
Sus scrofa
-
spleen
-
Bos taurus
-
spleen
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
assay procedure Gallus gallus
additional information
-
assay procedure Homo sapiens
additional information
-
assay procedure Rattus norvegicus
additional information
-
assay procedure Sus scrofa
additional information
-
assay procedure Bos taurus
additional information
-
assay procedure Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heme + 2 NADPH + 2 H+ + 2 O2 involved in heme metabolism Gallus gallus biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?
heme + 2 NADPH + 2 H+ + 2 O2 involved in heme metabolism Homo sapiens biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?
heme + 2 NADPH + 2 H+ + 2 O2 involved in heme metabolism Rattus norvegicus biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?
heme + 2 NADPH + 2 H+ + 2 O2 involved in heme metabolism Sus scrofa biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?
heme + 2 NADPH + 2 H+ + 2 O2 involved in heme metabolism Bos taurus biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?
heme + 2 NADPH + 2 H+ + 2 O2 involved in heme metabolism Oryctolagus cuniculus biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
?
heme + electron donor + O2 iron-protoporphyrin IX is the most active substrate, lower activity with: iron-mesoporphyrin IX, iron-deuteroheme IX, iron-coproheme I, alpha and beta chain of hemoglobin, poor substrates: oxyhemoglobin, carboxyhemoglobin, myoglobin Gallus gallus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 iron-protoporphyrin IX is the most active substrate, lower activity with: iron-mesoporphyrin IX, iron-deuteroheme IX, iron-coproheme I, alpha and beta chain of hemoglobin, poor substrates: oxyhemoglobin, carboxyhemoglobin, myoglobin Homo sapiens biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 iron-protoporphyrin IX is the most active substrate, lower activity with: iron-mesoporphyrin IX, iron-deuteroheme IX, iron-coproheme I, alpha and beta chain of hemoglobin, poor substrates: oxyhemoglobin, carboxyhemoglobin, myoglobin Rattus norvegicus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 iron-protoporphyrin IX is the most active substrate, lower activity with: iron-mesoporphyrin IX, iron-deuteroheme IX, iron-coproheme I, alpha and beta chain of hemoglobin, poor substrates: oxyhemoglobin, carboxyhemoglobin, myoglobin Sus scrofa biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 iron-protoporphyrin IX is the most active substrate, lower activity with: iron-mesoporphyrin IX, iron-deuteroheme IX, iron-coproheme I, alpha and beta chain of hemoglobin, poor substrates: oxyhemoglobin, carboxyhemoglobin, myoglobin Bos taurus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 iron-protoporphyrin IX is the most active substrate, lower activity with: iron-mesoporphyrin IX, iron-deuteroheme IX, iron-coproheme I, alpha and beta chain of hemoglobin, poor substrates: oxyhemoglobin, carboxyhemoglobin, myoglobin Oryctolagus cuniculus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 porphyrins without chelated iron and metalloporphyrins other than iron porphyrins are not oxidized Gallus gallus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 porphyrins without chelated iron and metalloporphyrins other than iron porphyrins are not oxidized Homo sapiens biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 porphyrins without chelated iron and metalloporphyrins other than iron porphyrins are not oxidized Rattus norvegicus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 porphyrins without chelated iron and metalloporphyrins other than iron porphyrins are not oxidized Sus scrofa biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 porphyrins without chelated iron and metalloporphyrins other than iron porphyrins are not oxidized Bos taurus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 porphyrins without chelated iron and metalloporphyrins other than iron porphyrins are not oxidized Oryctolagus cuniculus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
methemoglobin + electron donor + O2
-
Gallus gallus ?
-
?
methemoglobin + electron donor + O2
-
Homo sapiens ?
-
?
methemoglobin + electron donor + O2
-
Rattus norvegicus ?
-
?
methemoglobin + electron donor + O2
-
Sus scrofa ?
-
?
methemoglobin + electron donor + O2
-
Bos taurus ?
-
?
methemoglobin + electron donor + O2
-
Oryctolagus cuniculus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus
37
-
assay at Homo sapiens
37
-
assay at Rattus norvegicus
37
-
assay at Sus scrofa
37
-
assay at Bos taurus
37
-
assay at Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
4 several hours Gallus gallus
-
4 several hours Homo sapiens
-
4 several hours Rattus norvegicus
-
4 several hours Sus scrofa
-
4 several hours Bos taurus
-
4 several hours Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Rattus norvegicus