Literature summary for 1.14.14.17 extracted from

Lee, H.K.; Zheng, Y.F.; Xiao, X.Y.; Bai, M.; Sakakibara, J.; Ono, T.; Prestwich, G.D.
Photoaffinity labeling identifies the substrate-binding site of mammalian squalene epoxidase (2004), Biochem. Biophys. Res. Commun., 315, 1-9.

Search for more literature for 1.14.14.17

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
D407F	site-directed mutagenesis, mutant shows 8% of wild-type activity	Rattus norvegicus
K399F	site-directed mutagenesis, mutant shows 28% of wild-type activity	Rattus norvegicus
K399F/R400F/D407F	site-directed mutagenesis, triple mutant shows 10% of wild-type activity	Rattus norvegicus
K399P/R400P/D407P	site-directed mutagenesis, triple mutant shows 10% of wild-type activity	Rattus norvegicus
K399W/R400W/D407W	site-directed mutagenesis, inactive mutant	Rattus norvegicus
additional information	construction of a truncated enzyme mutant	Rattus norvegicus
R400F	site-directed mutagenesis, mutant shows 24% of wild-type activity	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
26-hydroxysqualene	competitive	Rattus norvegicus
NB-598	squalenoid noncompetitive allylamine inhibitor	Rattus norvegicus
trisnorsqualene alcohol	squalenoid noncompetitive inhibitor, mechanism-based inactivator	Rattus norvegicus
trisnorsqualene alcohol diazoester	i.e. TNSA-Dza, competitive, able to photocovalently modify the native protein	Rattus norvegicus
trisnorsqualene cyclopropylamine	squalenoid noncompetitive inhibitor, mechanism-based inactivator	Rattus norvegicus
trisnorsqualene hydroxylamine	mechanism-based inactivator	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	2 * 50000, recombinant truncated mutant enzyme, SDS-PAGE	Rattus norvegicus
100000	-	recombinant truncated mutant enzyme	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Rattus norvegicus

Reaction

Reaction	Comment	Organism	Reaction ID
squalene + [reduced NADPH-hemoprotein reductase] + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + [oxidized NADPH-hemoprotein reductase] + H2O	active site involves the key residues K399, R400, and D407	Rattus norvegicus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	location of the substrate-binding site by binding studies using the photolabeling inhibitor trisnorsqualene alcohol diazoester, key residues are K399, R400, and D407	Rattus norvegicus	?	-	?
squalene + reduced acceptor + O2	-	Rattus norvegicus	(S)-squalene-2,3-epoxide + acceptor + H2O	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 50000, recombinant truncated mutant enzyme, SDS-PAGE	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
SE	-	Rattus norvegicus
squalene epoxidase	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	inhibition kinetics	Rattus norvegicus
0.0184	-	trisnorsqualene alcohol diazoester	recombinant truncated enzyme mutant, pH 7.4, 37°C	Rattus norvegicus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)