Application | Comment | Organism |
---|---|---|
biotechnology | the CYP21 expression model system using resting Schizosaccharomyces pombe cells can be used for biotransformations | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene CYP21, expression in Schizosaccharomyces pombe strain CAD18, method optimization and evaluation, and quantification of the intracellular redox cofactor pool in transformed cells, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
17alpha-hydroxyprogesterone | inhibits above 0.2mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reaction kinetic, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a cytochrome P450 monooxygenase | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17-hydroxyprogesterone + NADPH + H+ + O2 | Homo sapiens | - |
11-deoxycortisol + NADP+ + H2O | - |
? | |
additional information | Homo sapiens | CYP21 performs the regio- and stereo-selective 21-hydroxylation of 17-alpha-hydroxyprogesterone when recombinantly expressed in Schizosaccharomyces pombe | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08686 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17-hydroxyprogesterone + NADPH + H+ + O2 | - |
Homo sapiens | 11-deoxycortisol + NADP+ + H2O | - |
? | |
17-hydroxyprogesterone + NADPH + H+ + O2 | the redox cofactor provides the electrons required for a P450-dependent hydroxylation. Maximal CYP 21 activity is observed in presence of low substrate concentrations of 0.2 mM. Higher substrate concentrations reduce the activity and inhibit the enzyme | Homo sapiens | 11-deoxycortisol + NADP+ + H2O | - |
? | |
additional information | CYP21 performs the regio- and stereo-selective 21-hydroxylation of 17-alpha-hydroxyprogesterone when recombinantly expressed in Schizosaccharomyces pombe | Homo sapiens | ? | - |
? | |
additional information | reaction method optimization, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP21 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | Cyp21 is a cytochrome P450 monooxygenase | Homo sapiens | |
NADPH | the redox cofactor provides the electrons required for a P450-dependent hydroxylation | Homo sapiens |