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Literature summary for 1.14.14.16 extracted from
- Purification and characterization of bovine steroid 21-hydroxylase (P450c21) efficiently expressed in Escherichia coli (2006), Biochem. Biophys. Res. Commun., 344, 400-405.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cymal 5 | optimal concentration is 0.002%, decrease in activity above 0.05% | Bos taurus |  |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | replacement of N-terminal membrane anchor and basic regions by the basic regions of CYP2C3 for efficient expression and purification. N-terminal membrane anchor and sequence of the basic region do not significantly affect either substrate-specificity or 21-hydroxylase activites | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0108 | - |
17alpha-hydroxyprogesterone | 37°C, pH 7.4, presence of 0.002% Cymal 5 | Bos taurus | |
| 0.0129 | - |
progesterone | 37°C, pH 7.4, presence of 0.002% Cymal 5 | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12400 | - |
gel filtration in presence of 0.12% Cymal 5, protein elutes as monomer surrounded by a micelle of detergent | Bos taurus |
| 53000 | - |
1 * 53000, calculated | Bos taurus |
| 80700 | - |
gel filtration in presence of 1% cholate, protein elutes as monomer surrounded by a micelle of cholate | Bos taurus |
| 167000 | - |
gel filtration in presence of 0.056% Cymal 6, protein elutes as monomer surrounded by a micelle of detergent | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | P00191 | expressed in Escherichia coli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17alpha-hydroxyprogesterone + NADPH + O2 | - |
Bos taurus | 17,21-dihydroxyprogesterone + NADP+ + H2O | - |
? | |
| progesterone + NADPH + O2 | - |
Bos taurus | deoxycorticosterone + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 53000, calculated | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| P450c21 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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