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Literature summary for 1.14.14.14 extracted from

  • Kellis, J.T.; Vickery, L.E.
    Purification and characterization of human placental aromatase cytochrome P-450 (1987), J. Biol. Chem., 262, 4413-20.
    View publication on PubMed
Search for more literature for 1.14.14.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00006
-
 androst-4-ene-3,17-dione pH 7.2, 37°C Homo sapiens
0.00021
-
 testosterone pH 7.2, 37°C Homo sapiens
0.058
-
 16alpha-hydroxytestosterone pH 7.2, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins Homo sapiens overall reaction estrone + formate + 4 H2O + 3 oxidized flavoproteins
-
 ?
testosterone + 3 O2 + 3 reduced flavoproteins Homo sapiens overall reaction 17beta-estradiol + formate + 4 H2O + 3 oxidized flavoproteins
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from microsome. Full enzymatic activity can be reconstituted with rabbit liver microsomal cytochrome P-450 reductase and Nonidet P-40 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
57
-
 pH 7.2, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
16alpha-hydroxytestosterone + 3 O2 + 3 reduced flavoprotein overall reaction Homo sapiens (16alpha,17beta)-estra-1(10),2,4-triene-3,16,17-triol + formate + 4 H2O + 3 oxidized flavoprotein
-
 ?
androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins overall reaction Homo sapiens estrone + formate + 4 H2O + 3 oxidized flavoproteins
-
 ?
testosterone + 3 O2 + 3 reduced flavoproteins overall reaction Homo sapiens 17beta-estradiol + formate + 4 H2O + 3 oxidized flavoproteins
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Homo sapiens

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 specific content of 11.5 nmol of P450/mg of protein. Enzyme displays spectroscopic properties typical of the ferric and ferrous forms of cytochrome P-450 Homo sapiens