Cloned (Comment) | Organism |
---|---|
expression of native and histidine-tagged versions of SMOA in Escherichia coli BL21 | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Benzene | competitive inhibitor of styrene | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
Styrene | pH 7.0, 37°C | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
styrene + FADH2 + O2 | Pseudomonas putida | - |
(S)-2-phenyloxirane + FAD + H2O | - |
? | |
styrene + FADH2 + O2 | Pseudomonas putida S12 | - |
(S)-2-phenyloxirane + FAD + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida S12 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
styrene + FADH2 + O2 | - |
Pseudomonas putida | (S)-2-phenyloxirane + FAD + H2O | - |
? | |
styrene + FADH2 + O2 | coupling of NADH and styrene oxidation can be best explained by a model, which includes both the direct transfer and passive diffusion of reduced FAD from NADH-specific flavin reductase (SMOB) to FAD-specific styrene epoxidase (SMOA) | Pseudomonas putida | (S)-2-phenyloxirane + FAD + H2O | - |
? | |
styrene + FADH2 + O2 | - |
Pseudomonas putida S12 | (S)-2-phenyloxirane + FAD + H2O | - |
? | |
styrene + FADH2 + O2 | coupling of NADH and styrene oxidation can be best explained by a model, which includes both the direct transfer and passive diffusion of reduced FAD from NADH-specific flavin reductase (SMOB) to FAD-specific styrene epoxidase (SMOA) | Pseudomonas putida S12 | (S)-2-phenyloxirane + FAD + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAD-specific styrene epoxidase | - |
Pseudomonas putida |
SMO | styrene monooxygenase (SMO) from Pseudomonas putida S12 is a two-component flavoenzyme composed of the NADH-specific flavin reductase, SMOB, and FAD-specific styrene epoxidase, SMOA | Pseudomonas putida |
SmoA | styrene monooxygenase (SMO) from Pseudomonas putida S12 is a two-component flavoenzyme composed of the NADH-specific flavin reductase, SMOB, and FAD-specific styrene epoxidase, SMOA | Pseudomonas putida |