Literature summary for 1.14.14.10 extracted from

Zhang, Y.; Edwards, T.E.; Begley, D.W.; Abramov, A.; Thompkins, K.B.; Ferrell, M.; Guo, W.J.; Phan, I.; Olsen, C.; Napuli, A.; Sankaran, B.; Stacy, R.; Van Voorhis, W.C.; Stewart, L.J.; Myler, P.J.
Structure of nitrilotriacetate monooxygenase component B from Mycobacterium thermoresistibile (2011), Acta Crystallogr. Sect. F, 67, 1100-1105.

Search for more literature for 1.14.14.10

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) Rosetta cells	Mycolicibacterium thermoresistibile

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.2 M magnesium chloride, 0.1 M MES pH 6.0 and 20% PEG 6000	Mycolicibacterium thermoresistibile

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium thermoresistibile	E5Q9D7	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap FF column chromatography and Superdex 75 gel filtration	Mycolicibacterium thermoresistibile

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrilotriacetate + FMNH2 + H+ + O2	-	Mycolicibacterium thermoresistibile	iminodiacetate + glyoxylate + FMN + H2O	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Mycolicibacterium thermoresistibile

Synonyms

Synonyms	Comment	Organism
nitrilotriacetate monooxygenase component B	-	Mycolicibacterium thermoresistibile
NTA-MoB	-	Mycolicibacterium thermoresistibile

Cofactor

Cofactor	Comment	Organism	Structure
FMNH2	-	Mycolicibacterium thermoresistibile

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)