Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Rosetta cells | Mycolicibacterium thermoresistibile |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.2 M magnesium chloride, 0.1 M MES pH 6.0 and 20% PEG 6000 | Mycolicibacterium thermoresistibile |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium thermoresistibile | E5Q9D7 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap FF column chromatography and Superdex 75 gel filtration | Mycolicibacterium thermoresistibile |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrilotriacetate + FMNH2 + H+ + O2 | - |
Mycolicibacterium thermoresistibile | iminodiacetate + glyoxylate + FMN + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Mycolicibacterium thermoresistibile |
Synonyms | Comment | Organism |
---|---|---|
nitrilotriacetate monooxygenase component B | - |
Mycolicibacterium thermoresistibile |
NTA-MoB | - |
Mycolicibacterium thermoresistibile |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Mycolicibacterium thermoresistibile |