Cloned (Comment) | Organism |
---|---|
expression of mutant H226Y in Escherichia coli | Oryctolagus cuniculus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant mutant enzyme H226Y in complex with bifonazole, X-ray diffraction structure determination and analysis at 2.3 A resolution | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
H226Y | site-directed mutagenesis, altered coordination of the Fe2+ ion and binding of inhibitor bifonazole | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bifonazole | an anti-fungal drug, complex formation with CYP2B4, inhibits monooxygenase activity and induces a type II binding spectrum in CYP2B4 mutant H226Y, plastic regions efficiently, because conformational changes are clustered, the 2B4-bifonazole dimer is different from the ligand-free 2B4dH dimer, which involves the coordination of His226 to the heme iron of the other monomer | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Oryctolagus cuniculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme iron, His226 is involved in metal ion coordination | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutant H226Y from Escherichia coli by ion exchange chromatography and ultracentrifugation | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-ethoxy-trifluoromethylcoumarin + [reduced NADPH-hemoprotein reductase] + O2 | O-deethylation | Oryctolagus cuniculus | ? | - |
? | |
additional information | regiospecific and stereo-specific activity, overview | Oryctolagus cuniculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Oryctolagus cuniculus |
Synonyms | Comment | Organism |
---|---|---|
CYP2B4 | - |
Oryctolagus cuniculus |
cytochrome P450 2B4 | - |
Oryctolagus cuniculus |