Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dimethylsulfoxid | up to 25% v/v | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
A74G/F88V | substrate specificity similar to wild-type | Bacillus subtilis |
A74G/F88V/S189Q | substrate spectrum much broader than for wild-type | Bacillus subtilis |
A74G/S189Q | substrate specificity similar to wild-type | Bacillus subtilis |
General Stability | Organism |
---|---|
not stable in NaCl above 150 mM | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
0.0065 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
0.0104 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
0.0245 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
0.0319 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
0.0618 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, wild-type | Bacillus subtilis |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | stable, and activity increases in presence of up to 25% DMSO (v/v) | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
isozyme CYP102A3, natural fusion protein consisting of a heme domain and a reductase domain | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-ionone + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
capric acid + [reduced NADPH-hemoprotein reductase] + O2 | about 10% of the activity with palmitic acid, wild-type | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
caprylic acid + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
naphthalene + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
octane + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q and for mutant A74G/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
omega-(p-nitrophenyl)decanoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
omega-(p-nitrophenyl)dodecanoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
omega-(p-nitrophenyl)octanoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
1.3 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
1.3 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
1.5 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
3.1 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
3.5 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis |