Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.92 extracted from

  • Fraaije, M.W.; Kamerbeek, N.M.; Heidekamp, A.J.; Fortin, R.; Janssen, D.B.
    The prodrug activator EtaA from Mycobacterium tuberculosis is a Baeyer-Villiger monooxygenase (2004), J. Biol. Chem., 279, 3354-3360.
    View publication on PubMed
Search for more literature for 1.14.13.92

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 2 mg7ml, 930% activation Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
3-acetyl-NADP+ 0.5 mM, 50% inhibition Mycobacterium tuberculosis
3-amino-NADP+ 0.05 mM, 74% inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 phenylacetone 25°C, pH 8.5 Mycobacterium tuberculosis
0.2
-
 2-dodecanone 25°C, pH 8.5 Mycobacterium tuberculosis
0.28
-
 phenylacetone 25°C, pH 8.5, in the presence of 0.006 mM bovine serum albumin Mycobacterium tuberculosis
0.34
-
 Ethionamide 25°C, pH 8.5 Mycobacterium tuberculosis
0.52
-
 benzylacetone 25°C, pH 8.5 Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated Mycobacterium tuberculosis 16020
-

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose, hydroxyapatite, Superdex 200 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-decanone + NADPH + O2
-
 Mycobacterium tuberculosis methyl nonanoate + octyl acetate + NADP+ + H2O
-
 ?
2-dodecanone + NADPH + O2
-
 Mycobacterium tuberculosis nonyl acetate + methyl decanoate + NADP+ + H2O
-
 ?
2-heptanone + NADPH + O2
-
 Mycobacterium tuberculosis pentyl acetate + NADP+ + H2O
-
 ?
2-hexanone + NADPH + O2
-
 Mycobacterium tuberculosis butyl acetate + NADP+ + H2O
-
 ?
2-octanone + NADPH + O2
-
 Mycobacterium tuberculosis heptyl acetate + NADP+ + H2O
-
 ?
3-octanone + NADPH + O2
-
 Mycobacterium tuberculosis ethyl hexanoate + pentyl propanoate + NADP+ + H2O
-
 ?
4-heptanone + NADPH + O2
-
 Mycobacterium tuberculosis propanyl butanoate + NADP+ + H2O
-
 ?
benzylacetone + NADPH + O2
-
 Mycobacterium tuberculosis ? + NADP+ + H2O
-
 ?
bicyclohept-2-en-6-one + NADPH + O2
-
 Mycobacterium tuberculosis 3-oxabicyclo[3.3.0]oct-6-en-2-one + NADP+ + H2O
-
 ?
ethionamide + NADPH + O2
-
 Mycobacterium tuberculosis ? + NADP+ + H2O
-
 ?
methyl-p-tolylsulfide + NADPH + O2
-
 Mycobacterium tuberculosis ? + NADP+ + H2O
-
 ?
phenylacetone + NADPH + H+ + O2
-
 Mycobacterium tuberculosis benzyl acetate + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
EtaA
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.017
-
 phenylacetone 25°C, pH 8.5 Mycobacterium tuberculosis
0.021
-
 benzylacetone 25°C, pH 8.5 Mycobacterium tuberculosis
0.023
-
 2-dodecanone 25°C, pH 8.5 Mycobacterium tuberculosis
0.027
-
 Ethionamide 25°C, pH 8.5 Mycobacterium tuberculosis
0.26
-
 phenylacetone 25°C, pH 8.5, in the presence of 0.006 mM bovine serum albumin Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Mycobacterium tuberculosis
NADPH
-
 Mycobacterium tuberculosis