Cloned (Comment) | Organism |
---|---|
fusion protein with glutathione-S-transferase | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | 70% inhibition with 0.1 M NaCl or KCl, competitive with respect to NADPH and non-competitive with respect to L-kynurenine | Rattus norvegicus | |
pyridoxal | less potent than pyridoxal phosphate | Rattus norvegicus | |
pyridoxal 5'-phosphate | non competitive inhibition | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial outer membrane | - |
Rattus norvegicus | 5741 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Rattus norvegicus |
55760 | - |
calculated from primary sequence | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
fusion protein with gluthatione-S-transferase | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.028 | - |
NADPH oxidase activity | Rattus norvegicus |
2 | - |
fusion protein with glutathione-S-transferase | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C stable for 6 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADPH + O2 | - |
Rattus norvegicus | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable for four hours | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
fusion protein with glutathione-S-transferase | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | about 50% of maximal activity at pH 7 and 9 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | one molecule non-covalently bound FAD per molecule of enzyme | Rattus norvegicus | |
NADH | less effective than NADPH | Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
1 | - |
pyridoxal | - |
Rattus norvegicus |