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Literature summary for 1.14.13.84 extracted from

  • Moonen, M.J.; Kamerbeek, N.M.; Westphal, A.H.; Boeren, S.A.; Janssen, D.B.; Fraaije, M.W.; van Berkel, W.J.
    Elucidation of the 4-hydroxyacetophenone catabolic pathway in Pseudomonas fluorescens ACB (2008), J. Bacteriol., 190, 5190-5198.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gne hapA, encoded in the hapCDEFGHIBA gene cluster, DNA and amino acid sequence determination and analysis Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71957
-
x * 71957, sequence calculation Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxyacetophenone + NADPH + H+ + O2 Pseudomonas fluorescens the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview 4-hydroxyphenyl acetate + NADP+ + H2O
-
?
4-hydroxyacetophenone + NADPH + H+ + O2 Pseudomonas fluorescens ACB the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview 4-hydroxyphenyl acetate + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens Q93TJ5 gene hapA, formerly hapE, encoded in the gene cluster hapCDEFGHIBA
-
Pseudomonas fluorescens ACB Q93TJ5 gene hapA, formerly hapE, encoded in the gene cluster hapCDEFGHIBA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyacetophenone + NADPH + H+ + O2
-
Pseudomonas fluorescens 4-hydroxyphenyl acetate + NADP+ + H2O
-
?
4-hydroxyacetophenone + NADPH + H+ + O2 the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview Pseudomonas fluorescens 4-hydroxyphenyl acetate + NADP+ + H2O
-
?
4-hydroxyacetophenone + NADPH + H+ + O2
-
Pseudomonas fluorescens ACB 4-hydroxyphenyl acetate + NADP+ + H2O
-
?
4-hydroxyacetophenone + NADPH + H+ + O2 the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview Pseudomonas fluorescens ACB 4-hydroxyphenyl acetate + NADP+ + H2O
-
?
additional information the enzyme is active with a wide range of aromatic and aliphatic ketones Pseudomonas fluorescens ?
-
?
additional information the enzyme is active with a wide range of aromatic and aliphatic ketones Pseudomonas fluorescens ACB ?
-
?

Subunits

Subunits Comment Organism
? x * 71957, sequence calculation Pseudomonas fluorescens

Synonyms

Synonyms Comment Organism
hAPA
-
Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Pseudomonas fluorescens