Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TOP10 | Pseudomonas fluorescens |
gene hapE, overexpression in Escherichia coli | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4-hydroxyphenyl)ethan-1-one + NADPH + O2 | Pseudomonas fluorescens | - |
4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
(4-hydroxyphenyl)ethan-1-one + NADPH + O2 | Pseudomonas fluorescens ACB | - |
4-hydroxyphenyl acetate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens | - |
gene hapE | - |
Pseudomonas fluorescens ACB | - |
- |
- |
Pseudomonas fluorescens ACB | - |
gene hapE | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4-hydroxyphenyl)ethan-1-one + NADPH + O2 | - |
Pseudomonas fluorescens | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
(4-hydroxyphenyl)ethan-1-one + NADPH + O2 | Baeyer-Villiger oxidation, stereospecific reaction, regioselectivity | Pseudomonas fluorescens | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
(4-hydroxyphenyl)ethan-1-one + NADPH + O2 | - |
Pseudomonas fluorescens ACB | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
(4-hydroxyphenyl)ethan-1-one + NADPH + O2 | Baeyer-Villiger oxidation, stereospecific reaction, regioselectivity | Pseudomonas fluorescens ACB | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
2-oxabicyclo[3.2.0]heptan-6-one + NADPH + O2 | - |
Pseudomonas fluorescens | ? | - |
? | |
2-oxabicyclo[3.2.0]heptan-6-one + NADPH + O2 | - |
Pseudomonas fluorescens ACB | ? | - |
? | |
2-oxabicyclo[4.2.0]octan-7-one + NADPH + O2 | - |
Pseudomonas fluorescens | ? | - |
? | |
3-oxabicyclo[3.2.0]heptan-6-one + NADPH + O2 | - |
Pseudomonas fluorescens | ? | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADPH + O2 | - |
Pseudomonas fluorescens | ? | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADPH + O2 | - |
Pseudomonas fluorescens ACB | ? | - |
? | |
bicyclo[3.2.0]heptan-6-one + NADPH + O2 | - |
Pseudomonas fluorescens | ? | - |
? | |
bicyclo[4.2.0]octan-7-one + NADPH + O2 | - |
Pseudomonas fluorescens | ? | - |
? | |
additional information | substrate specificity, regioselectivity, the enzyme preferably and stereospecifically catalyzes Baeyer-Villiger oxidations of of ketones bearing a cyclobutanone structural motif, e.g. oxidation of several prochiral cyclobutanones to antipodal butyrolactones, overview | Pseudomonas fluorescens | ? | - |
? | |
additional information | substrate specificity, regioselectivity, the enzyme preferably and stereospecifically catalyzes Baeyer-Villiger oxidations of of ketones bearing a cyclobutanone structural motif, e.g. oxidation of several prochiral cyclobutanones to antipodal butyrolactones, overview | Pseudomonas fluorescens ACB | ? | - |
? | |
tricyclo[4.2.1.02,5]nonan-3-one + NADPH + O2 | - |
Pseudomonas fluorescens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HAPMO | - |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas fluorescens | |
NADPH | - |
Pseudomonas fluorescens |