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Literature summary for 1.14.13.83 extracted from

  • Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.
    Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas denitrificans (1993), J. Bacteriol., 175, 7430-7440.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.83

Cloned(Commentary)

Cloned (Comment) Organism
genetic and sequence analysis Pseudomonas denitrificans (nom. rej.)

General Stability

General Stability Organism
upon handling or storing CobG preparations, the sulfur content, the A400, and the enzymatic activity drops simultaneously Pseudomonas denitrificans (nom. rej.)

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ iron-sulfur protein Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
precorrin-3A + NADH + H+ + O2 Pseudomonas denitrificans (nom. rej.) pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 precorrin-3B + NAD+ + H2O
-
 ir
precorrin-3A + NADH + H+ + O2 Pseudomonas denitrificans (nom. rej.) SC510 (RifT) pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 precorrin-3B + NAD+ + H2O
-
 ir
precorrin-3A + NADH + O2 Pseudomonas denitrificans (nom. rej.)
-
 precorrin-3B + NAD+ + H2O
-
 ?
precorrin-3A + NADH + O2 Pseudomonas denitrificans (nom. rej.) SC510 (RifT)
-
 precorrin-3B + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas denitrificans (nom. rej.)
-
-
-
Pseudomonas denitrificans (nom. rej.) SC510 (RifT)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas denitrificans (nom. rej.)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.048
-
-
 Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
precorrin-3A + NADH + H+ + O2
-
 Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O
-
 ir
precorrin-3A + NADH + H+ + O2 pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O
-
 ir
precorrin-3A + NADH + H+ + O2
-
 Pseudomonas denitrificans (nom. rej.) SC510 (RifT) precorrin-3B + NAD+ + H2O
-
 ir
precorrin-3A + NADH + H+ + O2 pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 Pseudomonas denitrificans (nom. rej.) SC510 (RifT) precorrin-3B + NAD+ + H2O
-
 ir
precorrin-3A + NADH + O2
-
 Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O
-
 ?
precorrin-3A + NADH + O2
-
 Pseudomonas denitrificans (nom. rej.) SC510 (RifT) precorrin-3B + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CobG
-
 Pseudomonas denitrificans (nom. rej.)