Literature summary for 1.14.13.83 extracted from

Scott, A.I.; Roessner, C.A.; Stolowich, N.J.; Spencer, J.B.; Min, C.; Ozaki, S.I.
Biosynthesis of vitamin B12. Discovery of the enzymes for oxidative ring contraction and insertion of the fourth methyl group (1993), FEBS Lett., 331, 105-108.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21DE3	Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	SDS-PAGE	Pseudomonas denitrificans (nom. rej.)
46990	-	predicted from the DNA sequence	Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
precorrin-3 + NADH + H+ + O2	Pseudomonas denitrificans (nom. rej.)	biosynthesis of vitamin B12, part of the ring contractase system for oxidative ring contraction	precorrin-3x + NAD+ + H2O	-	ir

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas denitrificans (nom. rej.)	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
precorrin-3 + NADH + H+ + O2	-	Pseudomonas denitrificans (nom. rej.)	precorrin-3x + NAD+ + H2O	-	ir
precorrin-3 + NADH + H+ + O2	biosynthesis of vitamin B12, part of the ring contractase system for oxidative ring contraction	Pseudomonas denitrificans (nom. rej.)	precorrin-3x + NAD+ + H2O	-	ir

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Release 2023.1 (January 2023)