Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | requirement for reconstituted system | Cucumis sativus | |
S-adenosyl-L-methionine | 1 mM, absolute requirement when magnesium-protoporphyrin IX is used as substrate | Cucumis sativus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methyl viologen | at micromolar concentrations | Cucumis sativus | |
methylene blue | at micromolar concentrations | Cucumis sativus | |
phenazine methosulfate | at micromolar concentrations | Cucumis sativus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Cucumis sativus | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 | Cucumis sativus | - |
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucumis sativus | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
reconstitution of the enzyme system from a high-speed supernatant and a membrane pellet | Cucumis sativus |
Storage Stability | Organism |
---|---|
-15°C, 30% loss of activity in supernatant, 70% loss of activity in pellet after 7 days | Cucumis sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX + NADPH + O2 | - |
Cucumis sativus | ? + NADP+ + H2O | - |
? | |
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 | - |
Cucumis sativus | 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O | - |
? | |
additional information | overview: tetrapyrrole with substituents at various positions | Cucumis sativus | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Cucumis sativus | |
NADPH | more effective than NADH below 2 mM, equally effective at 10 mM | Cucumis sativus |